Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

55 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of human articular osteoarthritic chondrocytes after stimulation with IL-1beta and p38 MAPK inhibition with SB203580 or Birb 796

ABSTRACT: Effect of stimulation with IL-1beta and p38 MAPK inhibition with SB203580 or Birb 796 on human articular osteoarthritic chondrocytes

ORGANISM(S): Homo sapiens

SUBMITTER: Joos Helga

PROVIDER: E-MEXP-1434 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Comparative genomic hybridization of Streptococcus mitis B6 and other Streptococcus mitis strains.

Project description:Genomic comparison of Streptococcus mitis B6 and other Streptococcus mitis strains.

2010-01-21 | E-MEXP-2497 | biostudies-arrayexpress

UNIL-intraspecific competition

Project description:Root and leaf transcriptomes of Arabidopsis plants growing alone or subjected to intraspecific competition

2012-12-06 | E-MEXP-3735 | biostudies-arrayexpress

shRNA profiling of human breast carcinoma cells expressing over 300 shRNA constructs using barcode tiling arrays - screen 1

Project description:RNAi screens via pooled short hairpin RNAs (shRNAs) have recently become a powerful tool for the identification of essential genes in mammalian cells. We synthesized DNA microarrays with six overlapping 25 nt long tiling probes complementary to each unique 60 nt molecular barcode sequence associated with every shRNA expression construct.. In this part of the study pooled shRNAs were transduced into MDA-MB-231 breast carcinoma cell lines and their inhibitory effects four weeks post transduction were analyzed via microarray hybridization.

2009-12-08 | E-MEXP-2476 | biostudies-arrayexpress

shRNA profiling of human breast carcinoma cells expressing over 300 shRNA constructs using barcode tiling arrays - screens 2 and 3

2009-12-08 | E-MEXP-2477 | biostudies-arrayexpress

shRNA profiling of human cells containing varying concentrations of shRNA expression constructs to determine shRNA abundance using barcodes

Project description:RNAi screens via pooled short hairpin RNAs (shRNAs) have recently become a powerful tool for the identification of essential genes in mammalian cells. We synthesized DNA microarrays with six overlapping 25 nt long tiling probes complementary to each unique 60 nt molecular barcode sequence associated with every shRNA expression construct. By analyzing dilution series of expression constructs we show how our approach allows quantification of shRNA abundance from a pool and how it clearly outperforms the commonly used analysis via the shRNAs half hairpin sequences. In this part of the study one equimolar reference pool and three test pools of varying template concentrations were PCR amplified and hybridized to individual arrays.

2009-12-08 | E-MEXP-2474 | biostudies-arrayexpress

shRNA profiling of human cells containing varying concentrations of shRNA expression constructs to determine shRNA abundance using half hairpin sequences

Project description:RNAi screens via pooled short hairpin RNAs (shRNAs) have recently become a powerful tool for the identification of essential genes in mammalian cells. We synthesized DNA microarrays with six overlapping 25 nt long tiling probes complementary to each unique 60 nt molecular barcode sequence associated with every shRNA expression construct. By analyzing dilution series of expression constructs we show how our approach allows quantification of shRNA abundance from a pool and how it clearly outperforms the commonly used analysis via the shRNAs half hairpin sequences. In this part of the study one equimolar reference pool and three test pools of varying template concentrations were PCR amplified and hybridized to individual arrays.

2009-12-08 | E-MEXP-2475 | biostudies-arrayexpress

Transcription profiling by array of Sordaria macrospora wild type strain undergoing sexual development and the mutant strains dSmtA-1 and dSmtA-2

Project description:Cross-species hybridizations of Sordaria macrospora targets on Neurospora crassa microarrays were performed with targets derived from the S. macrospora wild type undergoing sexual development (wt.sex) and the mutant strains dSmtA-1 and dSmtA-2. For each strain, two independent experiments were carried out with a dye switch in the second experiment. The following slide/target/dye combinations were done: slide N16-86: Cy3-target: wt.sex (1), Cy5-target: dSmtA-1 (1) slide N16-87: Cy3-target: dSmtA-1 (2), Cy5-target: dSmtA-2(2) slide N16-88: Cy3-target: dSmtA-2 (1), Cy5-target: wt.sex (2)

2010-04-29 | E-MEXP-2600 | biostudies-arrayexpress

Transcription profiling of human pancreatic cancer cells after treatment with nemorosone

Project description:Effects of several nemorosone concentrations on three pancreatic cancer cell lines with different degrees of differentiation were studied

2009-07-30 | E-MEXP-1869 | biostudies-arrayexpress

Transcription profiling by array of Medicago trunculata Tunisian inbred lines

Project description:12 individual inbred lines of Mtr were hybed to the Affymetrix Medicago GenChip

2010-04-30 | E-MEXP-2686 | biostudies-arrayexpress

Transcription profiling by array of human olfactory ecto-mesenchymal stem cells in familial dysautonomia

Project description:Exploration of transcriptome expression in 5 control and 4 familial dysautonomia (FD) human olfactory ecto-mesenchymal stem cells (hOE-MSCs) at very early (P1 and P2) and later (P5 and P9) cell passages.

2011-01-01 | E-MTAB-281 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data