Project description:Comparison of two different multiplex PCR primer pools in amplifying target HPV types from plasmid templates. Template concentrations are either 1 ng or 1 pg. Templates are detected by type-spcific LDR probes hybridized on microarray.
Project description:Comparison of two different multiplex PCR primer pools in amplifying target HPV types from plasmid templates. Template concentrations are either 10 pg or 100 pg. Templates are detected by type-spcific LDR probes hybridized on microarray.
Project description:The DNA content of bacteriophages from Bartonella grahamii was investigated by hybridization against cellular DNA from the same organism. Phage particles were isolated from plate grown bacteria as well as from different growth phases during culture in liquid medium.
Project description:A dilution series experiment to determine the sensitivity of a microarray method for human papillomavirus genotyping. HPV genotyping is based on multiplex PCR (PGMY-t primers) followed by ligation step where two probes are ligated together if a matching template is present in the mixture. The ligated probes are then detected on microarray. Plasmids containing the full genome of HPV 16 or 18 were used in the experiment. The concentrations were 1000, 100, 10 ,1, 0.1 and 0 femtograms of plasmid as a template in HPV PCR reaction.<br><br>
Project description:Arabidopsis thaliana wildtype (Col-0) was compared with the ntrc mutant under different photoperiods (short day: 8h/16h; long day 16h/8h light/dark) and different ages (10d and 28/21d).
Project description:The objectif was to investigated the anti-atherogenic effects of nutritional supplementation of NAR in different mouse models of hypercholesterolemia and use transcriptomic approach to identify its molecular targets in the liver and aorta.
Project description:At eight weeks of age, male mice were divided into two groups (15 mice per group) fed iso-energetic diet: a control-diet or the same diet supplemented with 0.2% of curcumin (Sigma Aldrich) for 16 weeks (Table S1). The dose of curcumin used in this study is equivalent to 1g curcumin consumption in humans, a level of consumption achievable in South-Asian countries. No significant difference in weight gain was observed between the two groups at the end of the experimental period (data not shown). After 16 weeks, mice were anaesthetized (40 mg pentobarbital/kg of body weight) and blood was collected from the abdominal aorta into heparinised tubes and plasma was stored at -80 C. After washing with sterilized PBS, aorta and heart samples were immediately frozen in liquid nitrogen and stored at -80 C. Original processed data are available in the archive: http://www.ebi.ac.uk/arrayexpress/files/E-MEXP-3185/E-MEXP-3185.additional.zip
Project description:1. Comparison of gene expression profiles of normal prostates and prostates isolated from 4-5 months old PSA-Cre;Pten-loxP/loxP mice<br>2.Comparison of the gene expression profile in the proximal and distal part of normal prostates
Project description:Roswell Park Human 19K Array CGH<br><br>the format of the data is:<br> <br>Reporter Identifier<br>Band<br>Count - number of replicate spots the data represents (some spots are excluded in image analysis, usually because they are too dim to be reliable)<br>chr - Chromosome<br>Start - Start location in base pairs<br>Stop - Stop Location in Base pairs<br>Center - Center in base pairs<br>g_loc - graphing location this is the location of the center in base pairs if the chromosomes were laid end to end<br>Log2 ratio - log2 ratio (test/control)<br>Ratio - linear version of the ratio above <br>A - Measure of brightness A=(log2 cy3 + log2 cy5)/2 <br>Flag - Used to color code spots<br> 3 - red probably mismapped<br> 4 - green potentially polymorphic<br> 5 - light blue Shows a high degree of duplication with other area in the genome (see UCSC genome browser) <br>reference - Reference for why the clone was flagged <br>Pub Med link - Pub Med ID for why clone was flagged