Transcription profiling of Plasmodium falciparum synchronized blood stages exposed to lethal doses of artesunate
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ABSTRACT:
Since artesunate is active on ring stages as well as on older trophozoite stages, we decided to explore different time points along the erythrocytic cycle and search for genes affected at each time point. Therefore, five drug treatment experiments, staggered between 20 hours and 30 hours of parasite development, were performed for the comparison of drug versus no drug at 90 minutes and 3 hours.
Different expression levels in artesunate-exposed vs. control unexposed cultures could reflect the added effects of the actual response to artesunate together with a possible difference in growth rate/developmental stage. To identify the developmentally regulated genes in the artesunate-free control culture, the 3 hours RNA was hybridized against the time 0 RNA (3 control experiments).
INSTRUMENT(S): Scanning hardware
ORGANISM(S): Plasmodium falciparum
SUBMITTER: Emmanuel BISCHOFF
PROVIDER: E-MEXP-1435 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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