Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

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Transcription profiling of female flounders isolated from different rivers


ABSTRACT: Comparison of environmentally sampled flounder from the Tyne and Alde estuaries, related experiment, E-MEXP-14

ORGANISM(S): Platichthys flesus

SUBMITTER: Timothy Williams 

PROVIDER: E-MEXP-15 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

A DNA expression array to detect toxic stress response in European flounder (Platichthys flesus).

Williams T D TD   Gensberg K K   Minchin S D SD   Chipman J K JK  

Aquatic toxicology (Amsterdam, Netherlands) 20031001 2


As a first stage in developing a DNA array-based approach to investigating the effects of pollutants on an environmentally relevant European fish species, we have constructed a 160-gene custom microarray for European flounder. Degenerate primers were used to amplify 110 different fragments of stress-related and other genes from European flounder cDNA and genomic DNA. Additionally, 22 fragments were obtained by suppressive subtractive hybridisation (SSH). These fragments were cloned and sequenced  ...[more]

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