Ontology highlight
ABSTRACT:
ORGANISM(S): Platichthys flesus
SUBMITTER: Timothy Williams
PROVIDER: E-MEXP-15 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Williams T D TD Gensberg K K Minchin S D SD Chipman J K JK
Aquatic toxicology (Amsterdam, Netherlands) 20031001 2
As a first stage in developing a DNA array-based approach to investigating the effects of pollutants on an environmentally relevant European fish species, we have constructed a 160-gene custom microarray for European flounder. Degenerate primers were used to amplify 110 different fragments of stress-related and other genes from European flounder cDNA and genomic DNA. Additionally, 22 fragments were obtained by suppressive subtractive hybridisation (SSH). These fragments were cloned and sequenced ...[more]