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Chromatin immunopreciptitation of Drosophila DNA using antibodies against RMA polymerase II CTD repeats

ABSTRACT: Crosslinked chromatin from wild type flies was sonicated into small fragments, and used in ChIP reactions using a monoclonal antibody, 8WG16, against RNA polymerase II CTD repeats. Input material was removed prior to chIP, and a control chIP was incubated with normal rabbit IgG. The Input and IPed DNA was purified, amplified, and hybridized to Affymetrix Drosophila genomic tiling arrays.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Cizhong Jiang

PROVIDER: E-MEXP-1520 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Nucleosome organization in the Drosophila genome.

Mavrich Travis N TN Jiang Cizhong C Ioshikhes Ilya P IP Li Xiaoyong X Venters Bryan J BJ Zanton Sara J SJ Tomsho Lynn P LP Qi Ji J Glaser Robert L RL Schuster Stephan C SC Gilmour David S DS Albert Istvan I Pugh B Franklin BF

Nature 20080413 7193

Comparative genomics of nucleosome positions provides a powerful means for understanding how the organization of chromatin and the transcription machinery co-evolve. Here we produce a high-resolution reference map of H2A.Z and bulk nucleosome locations across the genome of the fly Drosophila melanogaster and compare it to that from the yeast Saccharomyces cerevisiae. Like Saccharomyces, Drosophila nucleosomes are organized around active transcription start sites in a canonical -1, nucleosome-fre ...[more]

PMID: 18408708

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