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Genome wide profiling of PAX8 binding stes in PCCL3 rat cells

ABSTRACT: Analysis of PAX8 binding sites by chromatin immunoprecipitation coupled with ultra-high-throughput sequencing (ChIP-seq) in PCCL3 rat cells. Results provide insight into the contribution of this regulatory factor to transcription genome-wide. We generate a genome wide map of PAX8 binding sites in PCCL3 cells using as negative control an input DNA obtained just prior to Pax8 immunoprecipitation

ORGANISM(S): Rattus norvegicus

SUBMITTER: Pilar Santisteban

PROVIDER: E-GEOD-26871 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genome-wide analysis of Pax8 binding provides new insights into thyroid functions.

Ruiz-Llorente Sergio S Carrillo Santa de Pau Enrique E Sastre-Perona Ana A Montero-Conde Cristina C Gómez-López Gonzalo G Fagin James A JA Valencia Alfonso A Pisano David G DG Santisteban Pilar P

BMC genomics 20120424

<h4>Background</h4>The transcription factor Pax8 is essential for the differentiation of thyroid cells. However, there are few data on genes transcriptionally regulated by Pax8 other than thyroid-related genes. To better understand the role of Pax8 in the biology of thyroid cells, we obtained transcriptional profiles of Pax8-silenced PCCl3 thyroid cells using whole genome expression arrays and integrated these signals with global cis-regulatory sequencing studies performed by ChIP-Seq analysis<h ...[more]

PMID: 22531031

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Rattus norvegicus

Project description:PAX8 transcriptional profiling in rat PCCL3 cells

| PRJNA142163 | ENA