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Transcription profiling of human Ecr293 cells transfected with ponasterone A to identify ETS-domain transcription factor Elk-1 target genes

ABSTRACT: ETS-domain transcription factor Elk-1 target genes identified using an inducible dominant-negative expression system. Here, we use a stably-transfected EcR293 cell line containing a ponasterone A

ORGANISM(S): Homo sapiens

SUBMITTER: Leo Zeef

PROVIDER: E-MEXP-1526 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Overlapping promoter targeting by Elk-1 and other divergent ETS-domain transcription factor family members.

Boros Joanna J O'Donnell Amanda A Donaldson Ian J IJ Kasza Aneta A Zeef Leo L Sharrocks Andrew D AD

Nucleic acids research 20091201 22

ETS-domain transcription factors play important roles in controlling gene expression in a variety of different contexts; however, these proteins bind to very similar sites and it is unclear how in vivo specificity is achieved. In silico analysis is unlikely to reveal specific targets for individual family members and direct experimental approaches are therefore required. Here, we take advantage of an inducible dominant-negative expression system to identify a group of novel target genes for the ...[more]

PMID: 19789270

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