RNAi knock-down of VSG in T. brucei for 1 or 3 days

ABSTRACT: As an additional strategy for investigating T. brucei transcriptome responsiveness, the mRNA of a highly important protein, the variant surface glycoprotein (VSG) the major surface protein in the bloodstream stage, was suppressed with RNAi. VSG RNAi results in arrest of cell cycle progression in the bloodstream stage. In addition, the mRNA of a highly important protein for endocytosis, the clathrin heavy chain (CLH), was suppressed with RNAi. CLH knockdown leads to a complete block to endocytosis. We hypothesized that if trypanosomes were able to sense alterations in trafficking and respond to these changes, then depletion of these ORFs by RNAi would be expected to elicit a response at the transcriptome level.

part 1: 2 biological replicates of SMB cells trans

ORGANISM(S): Trypanosoma brucei

SUBMITTER: Lila Koumandou 

PROVIDER: E-MEXP-1528 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress