Transcription profiling of T. brucei bloodstream forms cultured in different proportions of fetal bovine serum to investigate iron-sensing mechanisms

ABSTRACT: Bloodstream trypanosomes are sensitive to iron starvation, and upregulate ESAG6/7 transferrin receptor (TfR) mRNA and protein levels when placed in low transferrin or iron-depleted media, and adjust TfR expression to compensate for reduced endocytosis. Both observations suggest a specific transcriptional-level response resulting from an iron-sensing mechanism. The transcriptomes of BSFs cultured in different proportions of fetal bovine serum (FBS) were analysed.

part 1: 4 biological replicates of SMB cells grown with 10% FBS (normal conditions) and 4 replicates of SMB cells grown with 30% FBS, as well as dye swaps, were used.

part 2: 3 biological replicates of SMB cells grown with 10% FBS (normal conditions) and 3 replicates of SMB cells grown without FBS (but supplemented

ORGANISM(S): Trypanosoma brucei

SUBMITTER: Lila Koumandou 

PROVIDER: E-MEXP-1530 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress