Project description:PDR mutants , strain YYA100 (pdr1?::KanMx6, pdr3?::His3Mx6) from Mol Biol Cell. 2007 December; 18(12): 49324944, was grown to OD 1-1.5 in YEPD and hybridyzed to an expression array in one channel with a wild-type strain reference RNA on the other channel
Project description:Expression profile of meiosis in strain with Ndt80 inducible by addition of Estradiol (as in Carlile and Amon 2008), in three time points from addition of Estradiol.
Project description:U2OS cells were infected with shRNA vector targeting Ark5 or control vector, selected on puromycin for 48hrs, seeded on 5cm dishes at 20% confluency and harvested in triplicate 48hrs later