Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Butyrate-induced transcriptional changes in human colonic mucosa

ABSTRACT: Effect of butyrate enemas in humans on trancriptional data measured in biopsy samples from the distal colon

ORGANISM(S): Homo sapiens

SUBMITTER: Steven Vanhoutvin

PROVIDER: E-MEXP-1705 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Butyrate-induced transcriptional changes in human colonic mucosa.

Vanhoutvin Steven A L W SA Troost Freddy J FJ Hamer Henrike M HM Lindsey Patrick J PJ Koek Ger H GH Jonkers Daisy M A E DM Kodde Andrea A Venema Koen K Brummer Robert J M RJ

PloS one 20090825 8

<h4>Background</h4>Fermentation of dietary fiber in the colon results in the production of short chain fatty acids (mainly propionate, butyrate and acetate). Butyrate modulates a wide range of processes, but its mechanism of action is mostly unknown. This study aimed to determine the effects of butyrate on the transcriptional regulation of human colonic mucosa in vivo.<h4>Methodology/principal findings</h4>Five hundred genes were found to be differentially expressed after a two week daily butyra ...[more]

PMID: 19707587

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Project description:MicroRNAs (miRNAs) have emerged as important players in the regulation of T-cell functionality. However, comprehensive insight into the extent of age-related miRNA changes in T cells is lacking. We established miRNA expression patterns of CD45RO- naïve and CD45RO+ memory T-cell subsets isolated from peripheral blood cells from young and elderly individuals. Unsupervised clustering of the miRNA expression data revealed an age-related clustering in the CD45RO- T cells, while CD45RO+ T cells clustered based on expression of CD4 and CD8. Seventeen miRNAs showed an at least 2-fold up- or downregulation in CD45RO- T cells obtained from young as compared to old donors. Validation on the same and independent samples revealed a statistically significant age-related upregulation of miR-21, miR-223 and miR-15a. In a T-cell subset analysis focusing on known age-related phenotypic changes, we showed significantly higher miR-21 and miR-223 levels in CD8+CD45RO-CCR7- TEMRA compared to CD45RO-CCR7+ TNAIVE-cells. Moreover, miR-21 but not miR-223 levels were significantly increased in CD45RO-CD31- post-thymic TNAIVE cells as compared to thymic CD45RO-CD31+ TNAIVE cells. Upon activation of CD45RO- TNAIVE cells we observed a significant induction of miR-21 especially in CD4+ T cells, while miR-223 levels significantly decreased only in CD4+ T cells. Besides composition and activation, we showed a borderline significant increase in miR-21 levels upon an increasing number of population doublings in CD4+ T-cell clones. Together, our results show that ageing related changes in miRNA expression are dominant in the CD45RO- T-cell compartment. The differential expression patterns can be explained by age related changes in T-cell composition, i.e. accumulation of CD8+ TEMRA and CD4+ post thymic expanded CD31- T cells and by cellular ageing, as demonstrated in a longitudinal clonal culture model. MicroRNA profiling was performed in eight T cell subsets: CD4 naive (CD3+CD4+CD45RO-), CD8 naive (CD3+CD4-CD45RO-), CD4 memory (CD3+CD4+CD45RO+) and CD8 memory (CD3+CD4-CD45RO+) T cells derived from 5 healthy young and 5 healthy old participants.

Dataset Information

Butyrate-induced transcriptional changes in human colonic mucosa

Publications

Butyrate-induced transcriptional changes in human colonic mucosa.

Similar Datasets

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