Transcription profiling of Arabidopsis wild type Col-0 and hsf407 double knock out plants after heat stress
Ontology highlight
ABSTRACT:
ORGANISM(S): Arabidopsis thaliana
SUBMITTER: Wolfgang Busch
PROVIDER: E-MEXP-1725 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Similar Datasets
Project description:Microarray experiments of the abi5 seeds were performed by analyzing the samples obtained from two different alleles (both for wild-type and mutant). For the analysis of gene expression profiles in abi5-1 seeds, RNA from Ws seeds was used. For the analysis of gene expression profiles in abi5-7 seeds, RNA from Col seeds was used.
2009-04-01 | E-MEXP-1940 | biostudies-arrayexpress
Project description:Microarray experiments of the srk2d srk2e srk2i seeds were performed by analyzing the samples obtained from two independent growth conditions (both for wild-type and mutant).For the analysis of gene expression profiles in seeds of srk2d srk2e srk2i, RNA from Col (CS60000) seeds was used.
2009-04-01 | E-MEXP-1939 | biostudies-arrayexpress
Project description:Microarray experiments of the abi3 seeds were performed by analyzing the samples obtained from two different alleles (both for wild-type and mutant).For the analysis of gene expression profiles in abi3-1 seeds, RNA from Ler seeds was used. For the analysis of gene expression profiles in abi3-6 seeds, RNA from Col seeds was used.
2009-04-01 | E-MEXP-1941 | biostudies-arrayexpress
Project description:Regulation of carotenoid composition and shoot branching in Arabidopsis by a chromatin modifying histone methyltransferase, SDG8<br>Comparison of transcript profiles between wild type Columbia and ccr1 (carotenoid and chloroplast regulatory) mutant, which contains a mutation in At1g77300 (SDG8)
2009-10-01 | E-MEXP-1787 | biostudies-arrayexpress
Project description:Arabidopsis plants were grown in plastic pots filled with peat moss for 3 weeks (principal growth stage 1.07-1.08) under a 16 h light/8 h dark regimen (40 ± 10 ?mol photons/m2/s) at 22 C.Dehydration treatment: The 3-week-old plants were grown for 2 or 3 days without watering. To obtain accurate results, we carefully raised single plants in Petri dishes, each containing an equal amount of soil. Soil moisture contents were calculated from soil dry weight. Untreated; the soil moisture content was 84.3%. Under dehydration, on the second day, the soil moisture content was 51.1%. Under dehydration, on the third day, the moisture content was 11.6%.
2009-08-07 | E-MEXP-2174 | biostudies-arrayexpress
Project description:Microbe-associated molecular pattern (MAMP)-triggered immunity (MTI) is the first layer of molecular defense encountered by pathogens when they attempt to infect plants. MTI is dependent on cell surface pattern-recognition receptors (PRR) which act upstream of mitogen-activated protein kinase (MAPK) pathways. Genetic screening and mutant knock-out lines have largely contributed to our knowledge of MTI. However, genetic screening is confined to phenotype-causing mutations and scarcely enables the discovery of redundantly-acting proteins. We sought to discover protein components that contribute in MTI, using a phenotype-independent approach to discern nucleus-localized proteins after MTI induction. We report on the nuclear proteome of Columbia-0 (Col-0) and chitin elicitor receptor kinase 1 (cerk1) mutant plants 15 min after MTI induction. Our approach revealed that MAMP-treated cerk1 plants had many proteins in common with Col-0-treated plants following chitosan treatment. cerk1 plants also manifested several unique proteins that were absent from Col-0 plants when elicited with chitosan indicating that they also perceive chitosan. Detailed analysis of the identified proteins revealed a nuclear accumulation of transcriptional regulators and transcription factors, DNA-modifying enzymes, RNA-binding proteins and ribosomal proteins. No novel MAPKs were found although Receptor for activated C kinase 1, a scaffold protein involved in defense, and a nucleotide binding leucine-rich repeat protein, implicated in resistance to Leptosphaeria maculans, were discovered in the nucleus of chitosan-treated plants and absent from water-treated control plants.
2018-10-26 | PXD003821 | Pride
Project description:Gene expression profile of floral apicies between the homzygous and heterozygous MtNAM genotypes was compared.
2012-06-14 | E-MEXP-3480 | biostudies-arrayexpress
Project description:Two alfalfa varieties, 'Chilean' (M. sativa ssp. sativa var. Chilean, drought sensitive) and 'Wisfal' (M. sativa ssp. falcata var. Wisfal, drought tolerant), with contrasting water use efficiency were subjected to water withholding for 11 days followed by re-watering. Samples were taken for well-watered plants and plants after five, eight, eleven days of drought stress as well as plants after recovery for one day following drought stress. Roots and shoots were sampled and analyzed separately by expression profiling using Affymetrix Medicago GeneChip.
2011-08-29 | E-MEXP-3320 | biostudies-arrayexpress
Project description:In this experiment we used leaves from 6-week-old Arabidopsis SDH1-1/sdh1-1 mutant and Wt plants (Ws). The leaves were collected in the middle of light period.
2010-12-31 | E-MEXP-2992 | biostudies-arrayexpress
Project description:The wild grass Brachypodium distachyon has emerged as a model system for temperate grasses and biofuel plants. However, the global analysis of miRNAs, molecules known to be key for eukaryotic gene regulation, has been limited in B. distachyon to studies examining a few samples or that rely on computational predictions. Similarly an in-depth global analysis of miRNA-mediated target cleavage using Parallel Analysis of RNA Ends (PARE) data is lacking in B. distachyon. B. distachyon small RNAs were cloned and deeply sequenced from 17 libraries that represent different tissues and stresses. Using a computational pipeline, we identified 116 miRNAs including not only conserved miRNAs that have not been reported in B. distachyon, but also non-conserved miRNAs that were not found in other plants. To investigate miRNA-mediated cleavage function, four PARE libraries were constructed from key tissues and sequenced to a total depth of approximately 70 million sequences. The roughly 5 million distinct genome-matched sequences that resulted represent an extensive dataset to analyze small RNA-guided cleavage events. Analysis of the PARE and miRNA data provided experimental evidence for miRNA-mediated cleavage of 264 sites in predicted miRNA targets. In addition, PARE analysis revealed that differentially expressed miRNAs in the same family guide specific target RNA cleavage in a correspondingly tissue-preferential manner. B. distachyon miRNAs and target RNAs were experimentally identified and analyzed. Knowledge gained from this study should provide insights into the roles of miRNAs and the regulation of their targets in B. distachyon and related plants. Examination of various tissues and stresses in Brachypodium by high throughput sequencing for small RNA profiling and PARE (Parallel Analysis of RNA Ends)
2013-12-06 | E-GEOD-52441 | biostudies-arrayexpress