Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of strains of Paxillus involutus that show different abilities in formation of ectomycorrhizal associations with birch (Betula pendula)

ABSTRACT: A microarray analysis was conducted to investigate transcriptional differences between strains of Paxillus involutus that show different abilities in formation of ectomycorrhizal associations with birch (Betula pendula). Our goal was to identify genes that showed differential regulation from comparing incompatible (not mycorrhiza forming) (Nau) and compatible (mycorrhiza forming) strains (ATCC200175 and Maj) of P. involutus, in association with birch. The entire design involved 11 slides (ME01-M11), 13 labelled extracts, and 3 biological replicates (Rep1-Rep3). The experiment was designed as a simple loop (all-pairwise comparisons) of all three strains including dye-swap, biological and technical replication. Additional information: In our pre-processing of data, 4 channels were excluded for further analyses due to overall poor quality and are listed below. Slide ME03, Cy5(635nm) (strain Nau, Rep2) Slide ME04, Cy5(635nm) (strain Nau, Rep2) Slide ME06, Cy5(635nm) (strain Maj, Rep2) Slide ME09, Cy5(635nm) (strain Nau, Rep2)

ORGANISM(S): Ectomycorrhizal tissue: Paxillus involutus/Betula pendula

SUBMITTER: Antoine Le Quéré

PROVIDER: E-MEXP-179 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Divergence in gene expression related to variation in host specificity of an ectomycorrhizal fungus.

Le Quéré Antoine A Schützendübel Andres A Rajashekar Balaji B Canbäck Björn B Hedh Jenny J Erland Susanne S Johansson Tomas T Tunlid Anders A

Molecular ecology 20041201 12

Ectomycorrhizae are formed by mutualistic interactions between fungi and the roots of woody plants. During symbiosis the two organisms exchange carbon and nutrients in a specific tissue that is formed at the contact between a compatible fungus and plant. There is considerable variation in the degree of host specificity among species and strains of ectomycorrhizal fungi. In this study, we have for the first time shown that this variation is associated with quantitative differences in gene express ...[more]

PMID: 15548293

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Dataset Information

Transcription profiling of strains of Paxillus involutus that show different abilities in formation of ectomycorrhizal associations with birch (Betula pendula)

Publications

Divergence in gene expression related to variation in host specificity of an ectomycorrhizal fungus.

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