Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Paxillus involutus, Betula pendula mycorrhizal tissue at five different time points after initial contact between the symbionts

ABSTRACT: Mycorrhizal tissue was analyzed at five different time points after initial contact between the symbionts had occurred. The experimental design also included reference material grown axenically in parallel during the time course, with the purpose of detecting differential regulation of genes not related to the mycorrhizal interaction. For the mycorrhizal tissue, we sequentially and directly compared time points, including one loop-back hybridization, whereas the reference material was directly compared with mycorrhizal tissue within each time point. For the mycorrhizal material three biological replicates were included (Rep 1-3) whereas for the reference material only two were included (Rep 1 and 2), which equals 25 individual samples used. In total 40 separate labeling reactions and 20 microarray hybridizations were performed.

ORGANISM(S): Betula pendula

SUBMITTER: Tomas Johansson 

PROVIDER: E-MEXP-180 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Divergence in gene expression related to variation in host specificity of an ectomycorrhizal fungus.

Le Quéré Antoine A   Schützendübel Andres A   Rajashekar Balaji B   Canbäck Björn B   Hedh Jenny J   Erland Susanne S   Johansson Tomas T   Tunlid Anders A  

Molecular ecology 20041201 12

Ectomycorrhizae are formed by mutualistic interactions between fungi and the roots of woody plants. During symbiosis the two organisms exchange carbon and nutrients in a specific tissue that is formed at the contact between a compatible fungus and plant. There is considerable variation in the degree of host specificity among species and strains of ectomycorrhizal fungi. In this study, we have for the first time shown that this variation is associated with quantitative differences in gene express  ...[more]

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