Project description:Microarray analyses were performed on Arabidopsis thaliana plants overexpressing ERF6. In this way it was hoped that any changes in the Arabidopsis transcriptome caused by overexpression would provide clues as to the role this gene plays in vivo.<br>Arabidopsis thaliana plants were transformed with 35S::ERF6 or the empty vector pK2GW7 (used as a control). Total RNA was extracted from untreated 10-day old seedlings. Three separate arrays were performed (the RNA for each slide having been extracted from an independent biological replicate).
Project description:Comparison of N. gonorrhoeae nmb1650 knockout mutant and wild-type parent strains grown in capnoaerobic conditions (normal atmosphere +5% CO2)
Project description:Comparison of N. meningitidis nmb1650 knockout mutant and parental strains grown in capnoaerobic conditions (normal atmosphere +5% CO2)
Project description:Human pancreatic islets were isolated from pancreas of deceased donors by Ricordi's procedure and cultured in CMRL 1066 medium additioned with human albumin. EVs were isolated from conditioned medium derived from islet culture after isolation. Once isolated, RNA of islets and islet-derived EVs was extracted and analyzed for microRNA expression within 48 hours after isolation.
Project description:Microarray analyses were performed on Arabidopsis thaliana plants over-expressing either AtERF5 or AtERF6, which are both transcription factors of the ethylene response factor (ERF) family. In this way it was hoped that any changes in the Arabidopsis transcriptome caused by their over-expression would provide clues as to the role(s) these genes play in vivo.<br>Arabidopsis thaliana plants were transformed with either 35S::AtERF5, 35S::AtERF6 or the empty vector pK2GW7 (used as a control). Total RNA was extracted from untreated 10-day old seedlings. Three separate arrays were performed for each of the two over-expressors, (the RNA for each slide having been extracted from an independent biological replicate).
Project description:Microarray analyses were performed on Arabidopsis thaliana plants overexpressing ERF5. In this way it was hoped that any changes in the Arabidopsis transcriptome caused by overexpression would provide clues as to the role this gene plays in vivo.<br>Arabidopsis thaliana plants were transformed with 35S::ERF5 or the empty vector pK2GW7 (used as a control). Total RNA was extracted from untreated 10-day old seedlings. Three separate arrays were performed (the RNA for each slide having been extracted from an independent biological replicate).