Project description:To investigate whether and what miRNAs expression might be regulated by VSV (vesicular stomatitis virus?) challenge, we analyzed the miRNA expression profile of mouse primary peritoneal macrophages infected with VSV by using an array-based miRNA profiling. After the infection of VSV at MOI 10 for 48 h, the array revealed that many miRNAs were up-regulated in macrophages?
Project description:Employing a transcriptomic approach, the gene expression profiles of C57BL/6 and CBA macrophages, before and after L. amazonensis infection in vitro, were compared. These strains were selected due to their different degrees of susceptibility to this parasite.
Project description:HuR Deficient and Control, Bone Marrow Derived Macrophages stimulated with Lipopolysaccharides, from Salmonella enteritidis, 100ng/ml for 0, 2 and 6 hours. RNA from 2.000.000 cells was extracted with the RiboPure kit.
Project description:Control or Mef2c-deficient (cKO) B cells were treated with 5ug/ml anti-IgM for 0, 6, 12, 24, or 48 hours before harvesting RNA for analysis.