Project description:Arabidopsis rosette leaves were harvested from plants grown under <br><br>different photoperiods under 100 M-5mol photons m-2 s-1 at 20 M-0C. <br><br><br><br>In the first experiment plants grown under short day conditions 8L/16D (8 h light / 16 h dark) for 4 weeks were compared with plants <br><br>grown under long day (16L/8D) for 3 weeks.<br><br><br><br>In the second experiment plants grown under 12L/12D <br><br>for 2 weeks were compared with plants grown first 2 weeks under <br><br>12L/12D and then two days under short day (8L/16D) conditions.
Project description:Arabidopsis thaliana seeds after imbibition were inoculated in ½ MS medium supplemented with 0.8% agar and 1% sucrose. Once the plant material was uniformly germinated, the experimental conditions were applied. 5d old light-grown uniformly germinated seedlings were washed seven times with sterile water with last wash given by ½ MS liquid medium without sucrose to remove residual exogenous sugar and the plant material was kept in ½ MS liquid without sucrose in the dark for all subsequent steps. Cultures were shaken at 140 rpm at 22oC for 24 h and then 3 h treatment was given with liquid ½ MS without glucose and liquid ½ MS supplemented with BR (0.1 ?M EBR), glucose (3%), glucose (3%) + BR (0.1 ?M EBR). Seedlings were harvested after 3h and preceded for RNA isolation and Microarray analysis.
Project description:Arabidopsis thaliana wildtype (Col-0) was compared with the ntrc mutant under different photoperiods (short day: 8h/16h; long day 16h/8h light/dark) and different ages (10d and 28/21d).
Project description:To monitor gene expression changes pre floral transition and during early flower development col-0 and tps1-2 GVG::TPS1 (von Dijken, 2004) plants were grown under SD (8 hr light, 16 hr dark) for 21 days. Plants were then shifted to LD (16 hr light, 8 hr dark) conditions to induce flowering. RNA was isolated from micro-dissected apical tissue harvested 0 and 5 days after the shift to LD and double-stranded cDNA was synthesized. Biotinylated cRNA probes were prepared and hybridized to the Affymetrix ATH1 array in duplicate (biological replicates).
Project description:imbibed Col-0 seeds were sown on ? X MS medium supplemented with 0.8% agar and 1% sucrose. The plates were first exposed to continuous light for 12 h to stimulate germination and then wrapped with two layers of aluminum foil and placed in the growth chamber for 5 d. Once the plant material was uniformly germinated, the experimental conditions were applied. 5-d-old, dark-grown seedlings were washed seven times with sterile water followed by a wash with ? X MS liquid medium without sucrose to remove residual exogenous sugar and the plant material was kept in ? X MS liquid without sucrose in the dark for all subsequent steps. Cultures were shaken at 140 rpm at 22 C for 24 h and then treated with ? X MS without glucose or ½ X MS supplemented with BR (100 nM), glucose (3%), or glucose (3%) + BR (100 nM) for 3 h. Seedlings were harvested after 3h and preceded for RNA isolation and microarray analysis.
Project description:Gene expression profile in Arabidopsis stems of T-DNA insertion lines Atwrky12-1 and Atwrky12-2 were compared with Columbia wild-type plants at 45 days after germination.
Project description:Arabidopsis plants expressing DEX-inducible version of the developmental regulator FIL were exposed to DEX or a solution lacking DEX and changes in gene expression assessed after 4h and 8h using microarray analysis.