Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

39 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of mouse WT Erk1 and Erk2 deficient CD8 T cells

ABSTRACT: Comparison of unstimulated and in vitro activated wild type and Erk1 or Erk2 deficient CD8 T cells

ORGANISM(S): Mus musculus

SUBMITTER: Warren DSouza

PROVIDER: E-MEXP-1833 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

The Erk2 MAPK regulates CD8 T cell proliferation and survival.

D'Souza Warren N WN Chang Chiung-Fang CF Fischer April M AM Li Manqing M Hedrick Stephen M SM

Journal of immunology (Baltimore, Md. : 1950) 20081201 11

The magnitude of T cell responses is determined by proliferation and survival decisions made by the responding cells. We now demonstrate that the Erk MAPK pathway plays a critical role in these cell fate decisions within CD8 T cells. While Erk1 is dispensable for all aspects of CD8 T cell activation, Erk2 is required for the proliferation of CD8 T cells activated in the absence of costimulation. Surprisingly, Erk2 is not required for proliferation following the addition of a costimulatory signal ...[more]

PMID: 19017950

Similar Datasets

Transcription profiling by array of mouse naïve, antigen-experienced and Friend-virus specific CD4 TCRbeta transgenic cells

Project description:Gene expression profiling of naive, antigen-experienced and Friend virus-specific CD4 TCRbeta transgenic cells

2011-09-21 | E-MEXP-2950 | biostudies-arrayexpress

Expression data from mesothelial (LP9) and mesothelioma cells (HMESO) inhibited for extracellular-regulated kinase (ERK) 1, 2, or 5

Project description:Extracellular-regulated kinases (ERK1/2 and 5) are known to play important roles in growth and drug resistance of various cancers. Here we show roles of inhibition of ERK1, ERK2, or ERK5 on gene expression profiles of epithelioid malignant mesothelioma (MM) cells (HMESO). Untransfected human mesothelial cells (LP9), MM cells (HMESO) or HMESO cells stably transfected with shControl, shERK1, shERK2 , or shERK5 plasmids were used. Microarrays were performed on samples from 3 independent experiments. Each sample was analyzed on a separate array (3 independent biological replicates).

2010-05-08 | E-GEOD-21750 | biostudies-arrayexpress

Context-dependent regulatory mechanisms control site-specific information processing within the MEK/ERK module

Project description:The impact of the cellular context and receptor type on the dynamics of phosphorylation cycles in signaling pathways are unclear. We employ an unbiased approach to identify network alterations that explain phosphorylation dynamics of the MEK/ERK module in response to hepatocyte growth factor or interleukin-6, comparing primary hepatocytes with the immortalized cell line HaCaT. By combining quantitative mass spectrometry with dynamic modeling, we elucidate the network structure in primary hepatocytes. For HaCaT, our model predicts additional dual feedback regulation, which we experimentally identify as DUSP6 and paxillin. Model analyses reveal switch-like ERK phospho-form distribution profiles in primary hepatocytes that are severely altered in HaCaT, explaining threshold, sensitivity and saturation behavior. We apply our approach to human hepatocellular carcinoma samples and identify, as predicted, elevated threonine-phosphorylated ERK levels in the tumor. This suggests that the prevalence of the mono-phosphorylated ERK rather than the doubly phosphorylated ERK is indicative for dysregulated proliferation.

2016-02-08 | PXD002460 | Pride

Genomic Collaboration of Estrogen Receptor-Î± and ERK2 in Regulating Gene and Proliferation Programs

Project description:The nuclear hormone receptor, estrogen receptor-alpha (ERÎ±), and MAP kinases both play key roles in hormone-dependent cancers, yet their interplay and the integration of their signaling inputs remain poorly understood. In these studies, we document that estrogen-occupied ERÎ± activates and interacts with ERK2, a downstream effector in the MAPK pathway, resulting in ERK2 and ERÎ± colocalization at chromatin binding sites across the genome of breast cancer cells. KEYWORDS: siRNA knock-down, ligand treatment MCF-7 human breast adenocarcinoma cells were tranfected with control, ERK1 and ERK2 siRNA for 60 hours and treated with 0.1% EtOH (Vehicle) or 10 nM E2 for 4 hours or 24 hours, and cDNA microarray analyses were carried out using Affymetrix [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array.

2011-01-10 | E-GEOD-24592 | biostudies-arrayexpress

Serratia_infections

Project description:Transcriptional responses in the gut of the main malaria vector Anopheles gambiae following oral bacterial infection with the entomopathogen Serratia marcescens were identified using DNA microarrays. S. marcescens is a common member of the mosquito gut microbiota, found in both laboratory reared and field collected mosquitoes, that can be potentially pathogenic as in Drosophila (Nehme et al., 2007), while it has been shown to influence the outcome of Plasmodium infections (Bando et al., 2013). S. marcescens belongs to the Enterobacteriaceae family, members of which have been shown to influence malaria transmission dynamics (Cirimotich et al., 2011, Boissiere et al., 2012). To further investigate the interactions between S. marcescens and the mosquito host, likely to shape, directly or indirectly, malaria transmission dynamics, An. gambiae mosquitoes, from the recently established N'gousso M form laboratory colony that retains much of the genetic variation of field mosquitoes, were antibiotic treated for 5 days and subsequently orally infected with the Db11-GFP strain of S. marcescens. Bacteria-fed mosquitoes were selected 2 days post infection, and, 3 days post infection, guts from bacteria-fed mosquitoes were dissected. Uninfected control mosquitoes were treated in the same way. Differential expression in the gut of S. marcescens infected mosquitoes, compared to uninfected controls, was identified by hybridizing labelled complementary RNA, derived from total RNA extracted from the respective gut pools, in customized Agilent 4x44k gene expression microarrays, comprising oligonucleotide probes encompassing all An. gambiae annotated genes of the AgamP3.6 release, with each probe represented in duplicate.

2013-11-30 | E-MEXP-3952 | biostudies-arrayexpress

Effect of LAP2alpha knockout in primary mouse myoblasts

Project description:To identify genes and molecular pathways directly or indirectly affected by the loss of LAP2? in skeletal muscle, gene expression profiles of primary Lap2?+/+ (n=3) and Lap2?-/- (n=3) neonatal<br><br>myoblasts were compared.

2010-05-26 | E-MEXP-2200 | biostudies-arrayexpress

Transcription profiling of Mus musculus liver cells to investigate effects of treatment with compound A B or C

Project description:Effects of furanone on global gene expression. CD-1mice were treated with compond A B or C for 21 days. The compoud was administered orally to each mouse using a gastric sonde.The researcher was not previously informed about wich compound was the control, this to ensure impartiality during the experiment and the outcoume of the results.

2009-11-12 | E-MEXP-1249 | biostudies-arrayexpress

Initiation of Invasion-Metastasis Cascade by DNp73

Project description:The experiment was designed to profile the transcriptome of human melanoma cell lines that differ in their delta ex2/3p73 expression. The differential gene expression of SK-Mel-29 stably overexpressing delta ex2/3p73-cDNA (SK-Mel-29.DNp73) was analyzed in comparison to parental cells with integrated empty vector . The DNp73-related gene expression signature was compared to the transcriptome of SK-Mel-103 and SK-Mel-147 cells with high endogenous DNp73 expression.

2014-05-01 | E-MEXP-3911 | biostudies-arrayexpress

Transcription profiling of Drosophila allowed to evolve a defense response to infection to Pseudomonas aeruginosa over 10 generations

Project description:Drosophila harbor substantial genetic variation for antibacterial defense. We allowed wild-caught Drosophila melanogaster to evolve a defense response to systemic infection with the human opportunistic pathogen, Pseudomonas aeruginosa over 10 generations. We performed genome wide transcriptional profiling in selected lines relative to control lines (not infected, but were exposed to the same bottleneck in population size as their paired selected lines by randomly selecting a set of individuals to found the next generation, and then infected at the end of the 10 generations), to identify specifically the genetic basis of the evolved immune response.

2009-02-20 | E-MEXP-2054 | biostudies-arrayexpress

Homeodomain dependent gene expression in HOX transformed primary cells

Project description:The gene expression profile was determined in primary cells transformed by two different HOX genes (HOXA1 and HOXA9) and by two HOX derivatives where the homeodomain was exchanged.

2013-01-31 | E-MEXP-3617 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data