Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

59 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling time series of S. aureus grown in mixed culture with L. lactis

ABSTRACT: the transcriptomic profile of S. aureus during mixed cultures with L. lactis was established on chemically defined medium at constant pH (6.6).

ORGANISM(S): Staphylococcus aureus

SUBMITTER: Sergine Even

PROVIDER: E-MEXP-1837 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Staphylococcus aureus virulence expression is impaired by Lactococcus lactis in mixed cultures.

Even Sergine S Charlier Cathy C Nouaille Sébastien S Ben Zakour Nouri L NL Cretenet Marina M Cousin Fabien J FJ Gautier Michel M Cocaign-Bousquet Muriel M Loubière Pascal P Le Loir Yves Y

Applied and environmental microbiology 20090508 13

Staphylococcus aureus is responsible for numerous food poisonings due to the production of enterotoxins by strains contaminating foodstuffs, especially dairy products. Several parameters, including interaction with antagonistic flora such as Lactococcus lactis, a lactic acid bacterium widely used in the dairy industry, can modulate S. aureus proliferation and virulence expression. We developed a dedicated S. aureus microarray to investigate the effect of L. lactis on staphylococcal gene expressi ...[more]

PMID: 19429556

Similar Datasets

Transcription profiling by array of Staphylococcus aureus grown in cheese matrix in mixed culture with Lactococcus lactis

Project description:Effect of the presence of Lactococcus lactis on Staphylococcus aureus transcriptome in cheese matrix. S. aureus was co-cultured with L. lactis LD61 in cheese matrix during 7 days. RNA samples were extracted at different time points (6 h, 8 h, 10 h, 24 h and 7 days) in order to monitor the dynamic response of S. aureus MW2 in cheese matrix in presence of L. lactis

2011-07-01 | E-MEXP-2842 | biostudies-arrayexpress

Transcription profiling by array of S. aureus MW2 in a cheese matrix

Project description:profile of expression of S aureus MW2 in model cheese matrix over a 7-days period.

2011-02-15 | E-MEXP-2617 | biostudies-arrayexpress

Transcription profiling of Arabidopsis thaliana ematode induced early giant cells and normalvascular root cells.

Project description:Giant cells (GCs)formed by meloidogyne javanica 3 days post infection were isolated by microaspiration from the Arabidopsis Col0 galls. Three independent biological replicates were used for hybridisation. The controls for comparisons were vascular cylinder tissues from uninfected root segments at equivalent positions to the galls in the infected roots. RNA extracted directly from either the controls or the GCs were amplified two times with a RNA polimerase linear amplification based approach

2010-07-27 | E-MEXP-2300 | biostudies-arrayexpress

Transcription profiling of rat heart transplants from Lewis to Lewis and Lewis to F344 strains with and without cold storage / prolonged cold ischemia.

Project description: Annual heart allograft failure in humans rates about 3-5%. The main reason after the first postoperative year is chronic rejection. Myointimal hyperplasia, the hellmark of chronic rejection, results in a specific type of ischemic heart disease. The lack of angina pectoris symptoms allow ventricular arrythmias, sudden cardiac death or heart failure to occur without warning. In addition, diagnostic tools such as endomyocardial biopsy, coronary angiography or intracoronary ultrasound fail to predict the individual risk for myocardial dysfunction. The mechanisms responsible for chronic rejection are predominantly alloimmune mediated with activated T cells, macrophages, B cell mediated antibody formation and secreted cytokines responding to HLA and other endothelial cell antigens. In addition, non immunologic risk factors such as recipient age, metabolic factors, hypertension and ischemia contribute to development of this disease. Previous studies have demonstrated that ischemia has a profound influence on short term allograft survival but the underlaying mechanisms remain largely unknown. Apoptosis seems to play a crucial role in ischemia/reperfusion injury and several mechanisms for programmed cell death have been described. However, consequences on long term cell function of viability have not been investigated. The aim of this study was to investigate the implication and the mechanism of prolonged cold organ storage as a non immunologic risk factor in the pathogenesis of chronic rejection in a cardiac allograft model. We aimed for answering the following specific questions: How does cold ischemia affect the alloimmue response short and long term? How does prolonged cold ischemia affect gene expression at later time points after transplantation? Does it influence gene expression during chronic rejection?

2010-10-13 | E-MEXP-2301 | biostudies-arrayexpress

Transcription profiling of mouse heart tissue from animals exposed to mainstream tobacco smoke

Project description:Male C57B1/CBA mice were exposed to mainstream tobacco smoke (MTS) from two cigarettes daily, 5 days/week for 6 or 12 weeks. Mice were sacrificed immediately, or six weeks following, the last cigarette. High density DNA microarrays were used to characterize global gene expression changes in whole heart. Western blot and ELISA assays were used to determine levels of Cyp1A1 protein in heart microsomes and total and active PAI-1 protein from tissue extracts.

2008-12-25 | E-MEXP-1553 | biostudies-arrayexpress

RNA immunoprecipitation of Trypanosoma cruzi to study messenger ribonucleoproteins (mRNPs) containing TcDhh1.

Project description:To study the composition of mRNPs containing TcDhh1, we carried out immunoprecipitation assays with anti-TcDhh1 and epimastigotes lysates. Pre-immune serum was used as control. We also carried out a ribonomic approach to identify the mRNAs present within the TcDhh1 immunoprecipitated complexes. For this purpose, competitive microarray hibridizations were performed against negative controls, the non-precipitated fraction.

2010-10-31 | E-MEXP-2448 | biostudies-arrayexpress

Transcription profiling of Vitis vinifera cv. Cabernet-Sauvignon leaves obtained from plants grown in different enviroments and infected with vascular ascomycete fungus Eutypa lata.

Project description:Eutypa dieback is a vascular disease that may severely affect vineyards throughout the world. In the present work, microarrays analysis were made in order (i) to improve our knowledge of grapevine (Vitis vinifera cv. Cabernet-Sauvignon) responses to Eutypa lata, the causal agent of Eutypa dieback and (ii) to identify genes that may prevent symptom development. Qiagen/Operon grapevine microarrays bearing 14,500 probes were used to compare between three experimental conditions (in vitro, greenhouse, vineyard), foliar material of infected symptomatic plants (S+R+), infected asymptomatic plants (S-R+), and healthy plants (S-R-). These plants were characterized by symptoms notation after natural (vineyard) or experimental (in vitro, greenhouse) infection, re-isolation of the fungus located in the lignified parts, and the formal identification of E. lata mycelium by PCR. Semi-quantitative RT-PCR experiments were run to confirm the expression of some genes of interest in response to E. lata. Their expression profiles were also studied in response to other grapevine pathogens (E. necator, P. viticola, B. cinerea). (i) Five functional categories including metabolism, defense reactions, interaction with environment, transport and transcription were up-regulated in S+R+ plants compared to S-R- plants. These genes, which cannot prevent infection and symptom development, are not specific since they were also upregulated after infection by powdery mildew, downy mildew and black rot. (ii) Most of the genes that may prevent symptom development are associated with the light phase of photosynthesis. This finding is discussed in the context of previous data on the mode of action of eutypin and Eutypa secreted polypeptide fraction.

2010-03-01 | E-MEXP-2337 | biostudies-arrayexpress

Transcription profiling of Physcomitrella patens response to UV-B

Project description:comparison of gene expression between UV-B irradiated Physcomitrella patens gametophores with their non-irradiated controls in two different UV irradiation fields.

2010-04-28 | E-MEXP-2508 | biostudies-arrayexpress

Transcription profiling by array of Pseudomonas aeruginosa wild type, BphP and BphO strains.

Project description:The transcriptome of two different Pseudomonas aeruginosa mutant strains were compared to the Pseudomonas aeruginosa wild type strain in the stationary growth phase

2010-04-24 | E-MEXP-2606 | biostudies-arrayexpress

Comparative genomic hybridization of three-spine sticklebacks from baltic sea, pacific ocean, lake vattern and lake pulmanki

Project description:DNA tested on array for probe hybridization success

2009-08-21 | E-MEXP-2309 | biostudies-arrayexpress