Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of human adenocarcinoma cell line A549 over-expressing ELMOD2

ABSTRACT: ELMOD2 cDNA from the IMAGE clone 3897166 was cloned into the pDsRed-Monomer-N1 expression vector. ELMOD2 was overexpressed in the A549(adenomcarcinoma)cell line and cells transfected with empty vector were considered controls. Transcript profiles were compared with the Affymetrix Hgu133plus2 arrays. Three biological replicates of each condition were included.

ORGANISM(S): Homo sapiens

SUBMITTER: Sara Bruce

PROVIDER: E-MEXP-1838 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

ELMOD2, a candidate gene for idiopathic pulmonary fibrosis, regulates antiviral responses.

Pulkkinen Ville V Bruce Sara S Rintahaka Johanna J Hodgson Ulla U Laitinen Tarja T Alenius Harri H Kinnula Vuokko L VL Myllärniemi Marjukka M Matikainen Sampsa S Kere Juha J

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 20091204 4

Viral infections and abnormal host response are thought to cause epithelial injury in idiopathic pulmonary fibrosis (IPF). To understand IPF pathogenesis, we have used overexpression cell models and expression microarrays to discover genes networked with ELMO domain containing 2 (ELMOD2) gene genetically implicated in IPF. The identified pathways were confirmed in vitro, and ELMOD2 protein expression was characterized in tissue samples. Here 303 genes were significantly altered after ELMOD2 tran ...[more]

PMID: 19966137

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Project description:Phenotypic plasticity, the ability of one genotype to express different phenotypes in response to changing environmental conditions, is one of the most common phenomena characterising the living world and is not only relevant for the ecology but also for the evolution of species. Daphnia, the waterflea, is a textbook example for predator induced phenotypic plastic defences including changes in life-history, behaviour and morphology. However, the analysis of molecular mechanisms underlying these inducible defences is still in its early stages. We exposed Daphnia magna to chemical cues of the predator Triops cancriformis to identify key processes underlying plastic defensive trait formation. D. magna is known to develop an array of morphological changes in the presence of T. cancriformis including changes of carapace morphology and cuticle hardening. To get a more comprehensive idea of this phenomenon, we studied four different genotypes originating from habitats with different predation history, reaching from predator-free to temporary habitats containing T. cancriformis. We analysed the morphologies as well as proteomes of predator-exposed and control animals. Three genotypes showed morphological changes when the predator was present. Using a high-throughput proteomics approach, we found 294 proteins which were significantly altered in their abundance after predator exposure in a general or genotype dependant manner. Proteins connected to genotype dependant responses were related to the cuticle, protein synthesis and calcium binding whereas the yolk protein vitellogenin increased in abundance in all genotypes, indicating their involvement in a more general response. Furthermore, genotype dependant responses at the proteome level correlated well with local adaptation to Triops predation. Altogether, our study provides new insights concerning genotype dependant and general molecular processes involved in predator-induced phenotypic plasticity in D. magna.

Dataset Information

Transcription profiling of human adenocarcinoma cell line A549 over-expressing ELMOD2

Publications

ELMOD2, a candidate gene for idiopathic pulmonary fibrosis, regulates antiviral responses.

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