Ontology highlight
ABSTRACT:
Human neuroblastoma cell line SH-SY5Y is a thrice cloned (SK-N-SH -> SH-SY -> SH-SY5 -> SH-SY5Y) subline of the neuroblastoma cell line SK-N-SH which was isolated and established in 1970. This cell line has 47 chromosomes. The cells possess a unique marker comprised of a chromosome 1 with a complex insertion of an additional copy of a 1q segment into the long arm, resulting in trisomy of 1q. The cell lines used in this study are SHSY5Y transfected with the constructs pCEP-C99I45F, pCEP-C99V50F, pCEP-C99 wildtype or mock transfected with an empty vector. Independent cell clones of each transfected line were used to provide biological replicates.
Overexpressed C99 I45F is intracellularly cleaved resulting in high Abeta42, but low Abeta40 levels.
Overexpressed C99V50F is intracellularly cleaved resulting in low Abeta42, but high Abeta40 levels.
Overexpressed C99 wildtype is intracellularly cleaved resulting in medium Abeta42 and Abeta40 levels
Mock is the SHSY5Y cell line transfected with the empty vector pCEP (Invitrogen) as a negative control
ORGANISM(S): Homo sapiens
SUBMITTER: Markus Uhrig
PROVIDER: E-MEXP-1913 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
PloS one 20090826 8
Alzheimer's disease (AD) is characterized by neuronal degeneration and cell loss. Abeta(42), in contrast to Abeta(40), is thought to be the pathogenic form triggering the pathological cascade in AD. In order to unravel overall gene regulation we monitored the transcriptomic responses to increased or decreased Abeta(40) and Abeta(42) levels, generated and derived from its precursor C99 (C-terminal fragment of APP comprising 99 amino acids) in human neuroblastoma cells. We identified fourteen diff ...[more]