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Transcription profiling of Arabidopsis wild type or mp mutant seedlings treated with auxin

ABSTRACT: Comparison of mp mutant seedlings with transgenics carrying a dexamethasone-inducible GR-bodenlos gene. Seedlings were pretreated with control medium or medium containing dexamethasone, followed by a 1 hour auxin treatment, and expression profiles were compared using Affymetrix ATH1 arrays.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Dolf Weijers

PROVIDER: E-MEXP-1948 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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MONOPTEROS controls embryonic root initiation by regulating a mobile transcription factor.

Schlereth Alexandra A Möller Barbara B Liu Weilin W Kientz Marika M Flipse Jacky J Rademacher Eike H EH Schmid Markus M Jürgens Gerd G Weijers Dolf D

Nature 20100310 7290

Acquisition of cell identity in plants relies strongly on positional information, hence cell-cell communication and inductive signalling are instrumental for developmental patterning. During Arabidopsis embryogenesis, an extra-embryonic cell is specified to become the founder cell of the primary root meristem, hypophysis, in response to signals from adjacent embryonic cells. The auxin-dependent transcription factor MONOPTEROS (MP) drives hypophysis specification by promoting transport of the hor ...[more]

PMID: 20220754

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Transcriptional profiles between mp mutant seedlings and transgenics carrying the dexamethasone-inducible GR-bdl protein

2010-04-01 | GSE13881 | GEO

Transcriptional profiles between mp mutant seedlings and transgenics carrying the dexamethasone-inducible GR-bdl protein

Project description:In order to identify targets of the transcription factor AUXIN RESPONSE FACTOR5 / MONOPTEROS (ARF5/MP), we compared transcriptomes of mp-B4149 mutant seedlings (9 day-old) and seedlings carrying the dexamethasone-inducible version of the MP inhibitor protein BODENLOS (GR-bdl). Without dexamethasone (DEX) treatment, this line is identical to the wild-type, while DEX treatment leads to strong inhibition of ARF-dependent transcription. To remove all endogenous MP-inhibiting Aux/IAA proteins, we treated mp or GR-bdl seedlings during 1 hour with auxin (50 micromolar Indole-3-Acetic Acid), either with or without a pretreatment with 10 micromolar DEX for 1 hour. Genes that are activated by MP are expected to br downregulated in mp seedlings and in the GR-bdl line afer DEX treatment. We used biological duplicates for each of the three treatments. Keywords: Transcriptional profiling of transcription factor targets Mp mutant and GR-bdl seeds were germinated on solid 1/2MS media, and transferred to liquid medium after 9 days of horizontal growth. Duplicate cultures were generated for mp mutants, and quadruplicates for the GR-bdl line. 10 micromolar DEX was added to both mp cultures and to two of the GR-bdl cultures, and seedlings were gently shaken during 1 hour. Then, 50 micromolar IAA was added to all 6 cultures and seedlings were incubated with gentle shaking for another hour. Total RNA was isolated from the seedlings using a QIAgen RNeasy kit, and reverse transcription, in-vitro transcription, cRNA labling and fragemntation was done according to established methods (Schmid et al., Nature Genetics [2005] 37, 501-506). Labeled cRNA was hybridized to Arabidopsis ATH1 arrays.

2010-04-12 | E-GEOD-13881 | biostudies-arrayexpress

Transcription profiling of mouse muscle and bone marrow SP and MP cells

Project description:This study identified gene expression of Side Population (SP) and Main Population (MP) cells, isolated from adult murine skeletal muscle and Bone Marrow. Five different preparations of muscle SP, muscle MP, Bone marrw SP and Bone marrow MP cells were used as replicates. SUBMITTER_CITATION: Liadaki K, Kho AT, Sanoudou D, Schienda J, Flint A, Beggs AH, Kohane IS, Kunkel LM. Side population cells isolated from different tissues share transcriptome signatures and express tissue-specific markers. Exp Cell Res. 2005;303(2):360-74.

2007-10-11 | E-GEOD-1571 | biostudies-arrayexpress

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Transcription profiling of Arabidopsis seedlings expressing LEC2-Glucocorticoid Receptor fusion protein, induced with dexamethasone for 0, 1 or 4 hours

Project description:LEAFY COTYLEDON2 activity was induced via a LEC2-Glucocorticoid Receptor fusion protein in Arabidopsis seedlings. Experiment Overall Design: Transgenic plants carrying a 35S:LEC2-Glucocorticoid Receptor transgene were used for LEC2 induction. Activity was induced in 8 day old seedlings for 1 or 4 hours with Dexamethasone application. Controls include uninduced transgenic plants and non-transgenic plants treated with Dex. Experiment Overall Design: Two biological replicates per treatment.

2007-10-03 | E-GEOD-3959 | biostudies-arrayexpress

Transcription profiling of hy5 single mutant, hyh single mutant, hy5 hyh double mutant and wild type Arabidopsis seedlings of two different ecotypes and with or without auxin treatment

Project description:Arabidopsis thaliana seedlings comprising hy5 single mutant , hyh single mutant, hy5 hyh double mutant and wild-type plants were germinated in the dark on agar medium on usual Murashig and Skoog medium (pH 5.8) without sucrose. After 3 days of germination, etiolated seedlings were transfered to light conditions in liquid media similar to previous one but without agar . After 5 hours, auxin (10 micromolar IAA) was added to all treated samples except to controls (Mock treatment). all samples were harvested after one-hour treatment and frozen for further RNA extraction. Aim of the project was to detect impact of exogenous auxin treatment on each genotype

2006-11-08 | E-MEXP-715 | biostudies-arrayexpress

Expression data from Vehicle, MP and MP+CSE_Bu treated SD rats

Project description:Understanding the mechanism of action of CSE-Bu on gene level in osteogenic effect. We used microarray to detail the effect of MP and MP+CSE_Bu on gene expression in SD rats and identified various classes of genes that are either upregulated or down-regulated.

2020-12-31 | GSE143289 | GEO

Transcription profiling of Arabidopsis seedlings treated with auxin vs. controls

Project description:Global gene expression data from 7-day old light-grown liquid cultured seedlings treated with or without auxin (5ÂµM IAA) for 2 h.

2008-06-14 | E-GEOD-630 | biostudies-arrayexpress

Transcription profiling of Arabidopsis LEAFY mutant seedlings treated with dexamethasone and/or cycloheximide

Project description:Global analysis of gene expression in 9 day old LEAFY-GR, 35S::LFY or Landsberg erecta seedlings treated with the steroid dexamethasone and/or the protein synthesis inhibitor cycloheximide.

2007-07-12 | E-GEOD-911 | biostudies-arrayexpress

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