Project description:full human genome expression microarrays were used to analysis of gene expression profiles upon the induction of MSC osteogenic differentiation.
Project description:The analysis of the expression profile of the two component system BvrR/BvrS of the B. abortus 2308 making the comparison of the expression of the B. abortus 2308 and B. abortus 2308 BvrR- (mutant in the gen BvrR) whit the microarray of brucella melitensis (Brucearray)
Project description:A direct analysis of the global transcriptional profile of B. abortus cells grown in laboratory conditions was made. Thus, three independent biological replicas were made, and following the RNA isolation, samples were reverse transcribed in aRNA and labelled with Cy3 fluorescent dye. The use of a single dye labelling approach allowed us an estimation of the genes more abundantly expressed in Brucella under laboratory conditions. The evaluation experiment was composed of three slides yielding six measurements per gene (representing three biological replicates, since each gene is present twice on each slide) to confirm the reproducibility of the gene expression data. <br>The complete dataset was sorted by the normalized and corrected fluorescence intensity values.