Transcription profiling of Brucella abortus using an ORFeome-based Brucella whole-genome DNA microarray

ABSTRACT: A direct analysis of the global transcriptional profile of B. abortus cells grown in laboratory conditions was made. Thus, three independent biological replicas were made, and following the RNA isolation, samples were reverse transcribed in aRNA and labelled with Cy3 fluorescent dye. The use of a single dye labelling approach allowed us an estimation of the genes more abundantly expressed in Brucella under laboratory conditions. The evaluation experiment was composed of three slides yielding six measurements per gene (representing three biological replicates, since each gene is present twice on each slide) to confirm the reproducibility of the gene expression data.
The complete dataset was sorted by the normalized and corrected fluorescence intensity values.

ORGANISM(S): Brucella abortus

SUBMITTER: Cristina Viadas 

PROVIDER: E-MEXP-1887 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress