Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

56 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of Candida albicans after induction of tet-OP4 promoter-EFG1 fusion under normoxia

ABSTRACT: Transformants either containing pCS1 (strain CCS1) or pNIM as control (strain CCS2) were induced by doxycycline (40 ï¾µg/ml) for the indicated times (T0-T10, 0 to 10 min) in aerated hypha-inducing medium (10 % serum, 37 ï¾°C). For each time point, cDNA of CCS1 and CCS2 were co-hybridised to microarrays and ratios of expression were calculated for each gene. Each gene is represented by duplicate spots on the DNA microarrays.

ORGANISM(S): Candida albicans

SUBMITTER: Joachim Ernst

PROVIDER: E-MEXP-2117 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Transcription profiling by array of C. albicans genes after induction of tet-OP4 promoter-EFG1 fusion under hypoxia.

Project description:Transformants either containing pCS1 (strain CCS1) or pNIM as control (strain CCS2) were induced by doxycycline (40 ï¾µg/ml) for the indicated times (T0-T10, 0 to 10 min) in hypha-inducing medium (10 % serum, 37 ï¾°C) under a stream of 93.8 % nitrogen, 6 % CO2 and 0.2 % oxygen. For each time point, cDNA of CCS1 and CCS2 were co-hybridised to microarrays and ratios of expression were calculated for each gene. Each gene is represented by duplicate spots on the DNA microarrays.

2010-03-18 | E-MEXP-2119 | biostudies-arrayexpress

Transcription profiling of Candida albicans wild type and sch9 mutant grown under normoxia, hypoxia and hypoxia with CO2

Project description:sch9-mutant and wildtype grown under normoxi, hypoxia (four hours under 0,2% O2), or hypoxia with CO2 (0,2 % O2 and 6 % CO2)

2010-03-01 | E-MEXP-2407 | biostudies-arrayexpress

Transcription profiling of maize to identify gene expression changes after chilling for 4 hours (alarm phase)

Project description:This experiment evaluates quick (alarm) response to chilling in chilling-sensitive maize plants. Maize inbred line cm109 were grown in optimal conditions until third leaf was fully developed. At this stage plants were divided into three experimental variants: k0 - control plants, frozen at the beginning of daylight, k4 - control plants kept in the same conditions and frozen after 4 hours since beginning of daylight, c4 - plants kept in 14 deg. C for 4 hours since "dawn". At the mentioned moments, leaves were harvested and frozen in liquid nitrogen for RNA isolation.

2009-11-01 | E-MEXP-1875 | biostudies-arrayexpress

RNAi profiling by array of human HEp-2 cells with siRNA-mediated depletion of RNase MRP protein components

Project description:To identify mRNA substrates for the human RNase MRP complex, we examined the effects of siRNA-mediated depletion of RNase MRP (and RNase P) on the transcriptome of HEp-2 cells. The expression of two RNase MRP protein components, hPop1 and Rpp40, was knocked-down and after 48 hours mRNA was isolated from these cells as well as from cells transfected with an siRNA targeting the GFP mRNA (siEGFP), which was used as a control. The expression levels of mRNAs were analyzed on a genome-wide scale using 21K microarrays.

2011-04-01 | E-TABM-981 | biostudies-arrayexpress

Transcription profiling of human CD34+ derived neutrophil progenitors from umbilical cord blood, in response to Mek1 activation

Project description:analysis of differential gene expression in CD34+ derived neutrophil progenitors from umbilical cord blood, in response to Mek1 activation

2009-08-14 | E-TABM-754 | biostudies-arrayexpress

Transcriptional profiling of Caenorhabditis elegans whole-organism extract and subsequent co-immune precipitation of mRNA with the poly-A binding protein FLAG::PAB-1 specifically expressed in body wall muscle cells.

Project description:Tissue specific microarray data is obtained by generating a whole-organism extract and subsequent co-immune precipitation of mRNA with the poly-A binding protein FLAG::PAB-1 specifically expressed in body wall muscle cells (Roy et al. 2002). Different lines were compared: SV911 (control), SV912 (CYE-1/CDK-2AF), and SV985 (CYD-1/CDK-4). As reference total RNA was used.

2011-11-25 | E-TABM-886 | biostudies-arrayexpress

Transcription profiling of Candida albicans wild type and mnl1 cells after treatment with acetic acid

Project description:Wt vs mnl1 cells after 300 min treatment with 20 mM acetic acid in SC-pH3.0

2008-07-31 | E-MEXP-1641 | biostudies-arrayexpress

Transcription profiling by array of mouse amygdala after 7 or 14 days of alcohol intake

Project description:Amygdala gene regulation during escalation of alcohol intake in mice

2011-12-31 | E-TABM-956 | biostudies-arrayexpress

psg1-mutant transcript-profile

Project description:gRNA from psg1-mutant compared with gRND from wt-strain

2013-09-14 | E-MEXP-3976 | biostudies-arrayexpress

Transcription profiling of rat heart transplants from Lewis to Lewis and Lewis to F344 strains with and without cold storage / prolonged cold ischemia.

Project description: Annual heart allograft failure in humans rates about 3-5%. The main reason after the first postoperative year is chronic rejection. Myointimal hyperplasia, the hellmark of chronic rejection, results in a specific type of ischemic heart disease. The lack of angina pectoris symptoms allow ventricular arrythmias, sudden cardiac death or heart failure to occur without warning. In addition, diagnostic tools such as endomyocardial biopsy, coronary angiography or intracoronary ultrasound fail to predict the individual risk for myocardial dysfunction. The mechanisms responsible for chronic rejection are predominantly alloimmune mediated with activated T cells, macrophages, B cell mediated antibody formation and secreted cytokines responding to HLA and other endothelial cell antigens. In addition, non immunologic risk factors such as recipient age, metabolic factors, hypertension and ischemia contribute to development of this disease. Previous studies have demonstrated that ischemia has a profound influence on short term allograft survival but the underlaying mechanisms remain largely unknown. Apoptosis seems to play a crucial role in ischemia/reperfusion injury and several mechanisms for programmed cell death have been described. However, consequences on long term cell function of viability have not been investigated. The aim of this study was to investigate the implication and the mechanism of prolonged cold organ storage as a non immunologic risk factor in the pathogenesis of chronic rejection in a cardiac allograft model. We aimed for answering the following specific questions: How does cold ischemia affect the alloimmue response short and long term? How does prolonged cold ischemia affect gene expression at later time points after transplantation? Does it influence gene expression during chronic rejection?

2010-10-13 | E-MEXP-2301 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data