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Transcription profiling of human irradiated MOLT4 cells to measure measure of degradation rates by actinomycin D

ABSTRACT: MOLT4 cells in log phase (1x106 cells/ml) were irradiated with 4 Gy at room temperature, at a dose rate of 2.45 Gy/minute with a 137Cs ?-irradiator. After 4 hours, actinomycin D (5 ?M) was added to block transcription. Aliquots of culture were removed at hourly intervals and RNA was extracted (Trizol; Invitrogen). RNA and cRNA quantity and quality was determined by Nanodrop spectrophotometer and Bioanalyser 2100 (Agilent). Transcript levels were determined at different time points using Affymetrix Human U133A microarrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Efterpi Papouli

PROVIDER: E-MEXP-2179 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Dissection of a complex transcriptional response using genome-wide transcriptional modelling.

Barenco Martino M Brewer Daniel D Papouli Efterpi E Tomescu Daniela D Callard Robin R Stark Jaroslav J Hubank Michael M

Molecular systems biology 20091117

Modern genomics technologies generate huge data sets creating a demand for systems level, experimentally verified, analysis techniques. We examined the transcriptional response to DNA damage in a human T cell line (MOLT4) using microarrays. By measuring both mRNA accumulation and degradation over a short time course, we were able to construct a mechanistic model of the transcriptional response. The model predicted three dominant transcriptional activity profiles-an early response controlled by N ...[more]

PMID: 19920812

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