Unknown,Transcriptomics,Genomics,Proteomics

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ShRNA profiling of human T-ALL cell lines - inhibition of TAL1 and NKX3.1 in T-ALL

ABSTRACT: We decreased TAL1 and NKX3.1 proteins levels using lentiviral deliveries of shTAL1 and shNKX3.1 in TAL1 expressing human T-ALL cell lines. Growth curves of cell lines showed that reduced TAL1 and NKX3.1 expressions resulted in a first phase where the number of cells did not increase likewise the shCTL expressing cells. To identify TAL1 and NKX3.1 target genes involved in T-ALL cell growth, we performed gene expression profiling of cell lines expressing shTAL1, shNKX3.1 or shCTL after two and three days of culture.

ORGANISM(S): Homo sapiens

SUBMITTER: Linhda Couvelard

PROVIDER: E-MEXP-2197 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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ShRNA profiling of human T-ALL cell lines - inhibition of TAL1 and NKX3.1 in T-ALL

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