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Transcription profiling of Acinetobacter baumannii multi-drug resistant strain and a susceptible reference strain - Abefflux

ABSTRACT: Transcriptomic comparison of a multidrug resistant Acinetobacter baumannii strain and a susceptible reference strain.

ORGANISM(S): Acinetobacter baumannii

SUBMITTER: Sebastien Coyne

PROVIDER: E-MEXP-2254 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Screening and quantification of the expression of antibiotic resistance genes in Acinetobacter baumannii with a microarray.

Coyne Sébastien S Guigon Ghislaine G Courvalin Patrice P Périchon Bruno B

Antimicrobial agents and chemotherapy 20091102 1

An oligonucleotide-based DNA microarray was developed to evaluate expression of genes for efflux pumps in Acinetobacter baumannii and to detect acquired antibiotic resistance determinants. The microarray contained probes for 205 genes, including those for 47 efflux systems, 55 resistance determinants, and 35 housekeeping genes. The microarray was validated by comparative analysis of mutants overexpressing or deficient in the pumps relative to the parental strain. The performance of the microarra ...[more]

PMID: 19884373

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