Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptome analysis Enterococcus faecium during mid-exponential growth phase in presence and absence of ampicillin

ABSTRACT: Effect of 20 ug/ml ampicillin on gene expression of Enterococcus faecium E1162 during mid-exponential growth phase

ORGANISM(S): Enterococcus faecium

SUBMITTER: Xinglin Zhang

PROVIDER: E-MEXP-3564 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Genome-wide identification of ampicillin resistance determinants in Enterococcus faecium.

Zhang Xinglin X Paganelli Fernanda L FL Bierschenk Damien D Kuipers Annemarie A Bonten Marc J M MJ Willems Rob J L RJ van Schaik Willem W

PLoS genetics 20120628 6

Enterococcus faecium has become a nosocomial pathogen of major importance, causing infections that are difficult to treat owing to its multi-drug resistance. In particular, resistance to the β-lactam antibiotic ampicillin has become ubiquitous among clinical isolates. Mutations in the low-affinity penicillin binding protein PBP5 have previously been shown to be important for ampicillin resistance in E. faecium, but the existence of additional resistance determinants has been suggested. Here, we ...[more]

PMID: 22761597

Similar Datasets

Transcriptome wt vs mdxR mutant in E. faecium E1162 grown in BHI

Project description:Comparative transcriptome analysis of E. faecium E1162 and its isogenic mdxR deletion mutant during growth in BHI in mid-exponential phase (A660 = 0.3)

2013-10-09 | E-MEXP-3760 | biostudies-arrayexpress

Enterococcus faecium E1162 transcriptome 25 C vs 37 C

Project description:The transcriptome of Enterococcus faecium E1162 growing in Brain heart Infusion Broth was compared in the mid-exponential growth phase (A660 = 0.3) at 25 C and 37 C.

2013-09-01 | E-MEXP-3941 | biostudies-arrayexpress

Transcriptome wt vs mdxR mutant in E. faecium E1162 grown in M1 with maltose

Project description:Comparative transcriptome analysis of E. faecium E1162 and its isogenic mdxR deletion mutant during growth in M1 with maltose in mid-exponential phase (A660 = 0.3)

2013-10-09 | E-MEXP-3759 | biostudies-arrayexpress

Transcriptome of E.faecium E1162 under bile salts activation

Project description:This experiment is to investigate E.faecium E1162 transcriptome profiles under bile salts activation.

2013-10-10 | E-MEXP-3796 | biostudies-arrayexpress

AsrR regulon

Project description:Enterococcus faecium is an important opportunistic pathogen emerging in hospitals worldwide. We identified a new MarR family global regulator in E. faecium, named AsrR (antibiotic and stress response regulator). We phenotipically characterized key role for AsrR in E. faecium pathogenicity. The aim of the microarray-based experiments was to investigate the AsrR regulon in E. faecium. We constructed a mutant strain deleted for the asrR gene, we complemented the mutant and, finally, we observed genes expression in these strains in comparison with the wild-type strain. (The parental HM1070 was used to delete the asrR gene and to obtain the mutant strain. Then, the mutant strain was used to restore the asrR gene and to obtain the complemented strain.) This approach will allow us to identify the genes regulated by AsR to clarify its role in E. faecium.

2012-07-09 | E-MEXP-3528 | biostudies-arrayexpress

Reproducibility M-Tram of Enterococcus faecium strain E1162

Project description:Two independent aliquots containing approximately 10^7 cells from the transposon mutant of E. faecium E1162 were grown at 37 C for 20 hours in 20 ml of BHI broth. Genomic DNA was isolated from the two replicate cultures and used for the generation of cDNA. The cDNA samples were labeled with Cy3 and Cy5 respectively and hybridized to a microarray that was designed using the E. faecium E1162 genome sequence.

2012-11-02 | E-MEXP-3502 | biostudies-arrayexpress

Amino acid substitutions in ribosomal protein RpsU enable switching between high fitness and multiple-stress resistance in Listeria monocytogenes

Project description:A previously described low-fitness, high stress-resistant, variant of Listeria monocytogenes LO28 WT was subjected to an experimental evolution regime, selecting (in two parallel lines) for increased fitness in unstressed conditions. Evolved variants with increased fitness reverted to WT-like stress resistance. Whole genome sequencing and proteomics were used to identify differences between the ancestral and evolved strains.

2023-10-03 | PXD022732 | Pride

Proteomics analysis of Listeria monocytogenes single and double mutants with mutation rpsUG50C, ΔsigB, and ΔrsbV.

Project description:This study was conducted to evaluate which SigB regulator proteins participated in SigB activation of Listeria monocytogenes rpsUG50C mutants.

2024-02-28 | PXD045800 | Pride

Chicken microbiome LC - MS / MS

Project description:Bacteroidaceae are common gut microbiota members in all warm-blooded animals. However, if Bacteroidaceae are to be used as probiotics, the species selected for different hosts should reflect the natural distribution. In this study, we therefore evaluated host adaptation of bacterial species belonging to the family Bacteroidaceae. B. dorei, B. uniformis, B. xylanisolvens, B. ovatus, B. clarus, B. thetaiotaomicron and B. vulgatus represented human-adapted species while B. gallinaceum, B. caecigallinarum, B. mediterraneensis, B. caecicola, M. massiliensis, B. plebeius and B. coprocola were commonly detected in chicken but not human gut microbiota. There were 29 genes which were present in all human-adapted Bacteroides but absent from the genomes of all chicken isolates and these included genes required for the pentose cycle, and glutamate or histidine metabolism. These genes were expressed during an in vitro competitive assay, in which human-adapted Bacteroides species overgrew the chicken adapted isolates. Not a single gene specific for the chicken-adapted species was found. Instead, chicken adapted species exhibited signs of frequent horizontal gene transfer, of KUP, linA and sugE genes in particular. The differences in host adaptation should be considered when the new generation of probiotics for humans or chickens is designed.

2020-09-28 | PXD020954 | Pride

M-TraM analysis for identification of chlorhexidine resistance genes in Enterococcus faecium E1162

Project description:Microarray-based Transposon Mapping (M-TraM) of E. faecium E1162 to identify conditionally essential genes, required for growth in the presence of chlorhexidine (1.2 ug/ml)

2016-07-03 | E-MTAB-4173 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data