Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

43 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of Arabidopsis wild type, 35S-ARR7, arr7 and 35S-ARR15 treated with cytokinin, auxin or both

ABSTRACT: Above ground tissue of 10 day old Arabidopsis seedlings of Col wild-type, 35S-ARR7, arr7, 35S-ARR15 was treated with Cytokinin (benzyladenine), Auxin (indole-3-acetic acid) or both.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Jan Lohmann

PROVIDER: E-MEXP-2270 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Hormonal control of the shoot stem-cell niche.

Zhao Zhong Z Andersen Stig U SU Ljung Karin K Dolezal Karel K Miotk Andrej A Schultheiss Sebastian J SJ Lohmann Jan U JU

Nature 20100601 7301

The classic phytohormones cytokinin and auxin play essential roles in the maintenance of stem-cell systems embedded in shoot and root meristems, and exhibit complex functional interactions. Here we show that the activity of both hormones directly converges on the promoters of two A-type ARABIDOPSIS RESPONSE REGULATOR (ARR) genes, ARR7 and ARR15, which are negative regulators of cytokinin signalling and have important meristematic functions. Whereas ARR7 and ARR15 expression in the shoot apical m ...[more]

PMID: 20577215

Similar Datasets

The embryonic leaf identity gene FUSCA3 also regulates vegetative phase transitions by negatively regulating ethylene action in Arabidopsis

Project description:The embryonic temporal regulator FUSCA3 (FUS3) plays major roles in the establishment of embryonic leaf identity and regulation of developmental timing. Loss-of-function mutations of this B3-domain transcription factor result in replacement of cotyledons with leaves and precocious germination, while constitutive misexpression causes the conversion of leaves into cotyledon-like organs and delays vegetative and reproductive phase transitions. To identify downstream targets of FUS3 involved in vegetative phase transitions, we performed microarray analysis on seedlings that transiently activate FUS3 using the AtML1:FUS3-GR-DEX inducible system. Using this construct, FUS3 is ectopically expressed in the ML1 or epidermal layer and becomes activated upon addition of dexamethasone. We found that activation of FUS3 after germination dampens the expression of genes involved in the biosynthesis and response to the plant hormone ethylene, while a loss-of-function fus3 mutant shows many phenotypes consistent with increased ethylene signaling. This FUS3-dependent regulation of ethylene signaling also impinges on timing functions outside of embryogenesis. Loss of FUS3 function results in accelerated vegetative phase change and this is again partially dependent on functional ethylene action. This alteration in vegetative phase transition is dependent on both embryonic and vegetative FUS3 function, suggesting that this important transcriptional regulator controls both embryonic and vegetative developmental timing. This study indicates that the embryonic regulator FUS3 not only regulates the embryonic-to-vegetative phase transition through hormonal regulation, but also functions postembryonically to modulate vegetative phase transitions by negatively regulating ethylene action.

2011-12-11 | E-MEXP-3465 | biostudies-arrayexpress

Arabidopsis seedlings and cytokinin

Project description:Aim of this experiment was to look at the gene expression differences in the benzyladenine-treated and untreated Arabidopsis seedlings grown in light

2013-03-27 | E-MTAB-1596 | biostudies-arrayexpress

Arabidopsis seedling treated with BA and Zeatin

Project description:Aim was to look at the gene profile changes in 10 days old Arabidopsis seedlings grown in light and treated with either Zeatin or BA and compared to non-treatment

2013-03-28 | E-MTAB-1601 | biostudies-arrayexpress

Dark grown Arabidopsis seedlings treated with cytokinin

Project description:Arabidopsis seed lings were grown in dark for 10 days on MS media and treated with DMSO (control) or 1 µM of BA for either 45 or 120 minutes to look for changes in genes profiles

2013-03-28 | E-MTAB-1597 | biostudies-arrayexpress

Transcription profiling by array of Arabidopsis thaliana seedlings treated with CTL-VI or GR24

Project description:Effect of the chemical EL6 and GR24 on seedling growth in Arabidopsis

2010-01-06 | E-MEXP-2494 | biostudies-arrayexpress

Transcriptional profiling of DEX-inducible SNRK3.15 seedlings in the presence of ABA

Project description:Transcriptional profiling of a DEX-inducible SNRK3.15 seedlings in the presence of ABA.

2012-03-01 | E-MEXP-3397 | biostudies-arrayexpress

Transcription profiling by array of wild type and arr1,10,12 mutant Arabidopsis seedlings treated with the cytokinin benzyladenine

Project description:Effect of the cytokinin BA on wt and arr1,10,12 mutant seedlings

2008-06-01 | E-MEXP-1573 | biostudies-arrayexpress

Transcription profiling time series of Arabidopsis seedlings treated with the cytokinin benzyladenine or DMSO

Project description:10 day old seedlings were treated with 5uM of the cytokinin Benzyladenine(BA)or DMSO at 15min, 45min, 120min, 480min and 1440min

2006-08-24 | E-MEXP-749 | biostudies-arrayexpress

Transcription profiling of Arabidopsis seedlings of wild type, brx loss-of-function and rescued brx lines

Project description:We compared the seedling transcription profiles to determine the effects of loss-of-function of the BRX gene of Arabidopsis. BRX is required for optimal root growth. We compared seedlings of a loss-of-function line (brx) with its control background (Sav-0). Because the loss-of-function line was derived from introgression, a brx line that was complemented by a transgenic wild type copy of BRX was also included as a control. This line (rescued brx) allows the identification of expression differences that are due to introgression drag. See Mouchel et al. 2004, Genes & Dev. Vol. 18, p. 700 for a detailed description. We also compared to response of the different genotypes to the application of the phytohormones brassinolide (BL) and indole acetic acid (IAA)

2006-09-28 | E-MEXP-637 | biostudies-arrayexpress

Transcription profiling of Arabidopsis after induction of Golden2-like (GLK1 or GLK2) expression in a glk mutant background to identify downstream gene targets

Project description:Defining the transcriptome response to the brief induction of Golden2-like (GLK) gene expression in a glk mutant background. Expression of either GLK1 or GLK2 from an inducible promoter by the application of dexamethasone. Changes to transcriptome four hours after induction are compared with uninduced controls.

2009-02-01 | E-MEXP-1794 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data