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Transcription profiling of Francisella tularensis to spermine

ABSTRACT: Francisella tularensis LVS was grown in MH broth in the presence or absence of 200uM spermine. RNA was harvasted from overnight (16 hour) cultures and processed for microarray hybridization.

ORGANISM(S): Francisella tularensis

SUBMITTER: Paul Carlson

PROVIDER: E-MEXP-2327 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Global transcriptional response to spermine, a component of the intramacrophage environment, reveals regulation of Francisella gene expression through insertion sequence elements.

Carlson Paul E PE Horzempa Joseph J O'Dee Dawn M DM Robinson Cory M CM Neophytou Panayiotis P Labrinidis Alexandros A Nau Gerard J GJ

Journal of bacteriology 20090911 22

Tularemia is caused by the category A biodefense agent Francisella tularensis. This bacterium is associated with diverse environments and a plethora of arthropod and mammalian hosts. How F. tularensis adapts to these different conditions, particularly the eukaryotic intracellular environment in which it replicates, is poorly understood. Here, we demonstrate that the polyamines spermine and spermidine are environmental signals that alter bacterial stimulation of host cells. Genomewide analysis sh ...[more]

PMID: 19749055

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