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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptional response of Pseudomonas aeruginosa PAO1 to human host defense peptide LL-37

ABSTRACT: In this experiment the transcriptional response of the opportunistic human pathogen Pseudomonas aeruginosa towards physiological concentrations of the major human host defense peptide LL-37 was investigated using microarrays. To this aim, three independent cultures of P. aeruginosa PAO1 were grown until mid-log phase in Mueller-Hinton broth and subsequently incubated with either sublethal LL-37 concencentrations (20 M-5g/ml) or without peptide for 2 h at 37 M-0C following RNA extraction and microarray analysis.

ORGANISM(S): Pseudomonas aeruginosa

SUBMITTER: Nikola Strempel

PROVIDER: E-MEXP-3970 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Human host defense peptide LL-37 stimulates virulence factor production and adaptive resistance in Pseudomonas aeruginosa.

Strempel Nikola N Neidig Anke A Nusser Michael M Geffers Robert R Vieillard Julien J Lesouhaitier Olivier O Brenner-Weiss Gerald G Overhage Joerg J

PloS one 20131213 12

A multitude of different virulence factors as well as the ability to rapidly adapt to adverse environmental conditions are important features for the high pathogenicity of Pseudomonas aeruginosa. Both virulence and adaptive resistance are tightly controlled by a complex regulatory network and respond to external stimuli, such as host signals or antibiotic stress, in a highly specific manner. Here, we demonstrate that physiological concentrations of the human host defense peptide LL-37 promote vi ...[more]

PMID: 24349231

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