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Transcription profiling of Zea mays leaves from variety Helen grown in agricultural fields at 2 levels of nitrogen fertilization

ABSTRACT: Comparison of the transcriptome profiles of a widely commercialized maize variety (Helen) at two levels of nitrogen fertilization. Conventional nitrogen fertilization compared to not nitrogen fertilization during the season.

ORGANISM(S): Zea mays

SUBMITTER: Maria Pla

PROVIDER: E-MEXP-2367 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcription profiling of Zea mays leaves from variety MON810 (Helen Bt) grown in agricultural fields at 2 levels of nitrogen fertilization

Project description:Comparison of the transcriptome profiles of a widely commercialized MON810 maize variety (HelenBt) at two levels of nitrogen fertilization. Variety Helen Bt; obtained by Advanta; authorized the 11/08/2005; now commercialized by Limagrain Iberica. Conventional nitrogen fertilization compared to not nitrogen fertilization during the season.

2010-09-21 | E-MEXP-2368 | biostudies-arrayexpress

Transcription profiling of Zea mays leaves from MON810 (Helen Bt) and its near-isogenic couterpart (Helen) grown in agricultural fields

Project description:Comparison of the transcriptome profiles of a widely commercialized maize MON810 variety and its non-GM near-isogenic counterpart grown in agricultural fields. The Helen variety was commercialized by Limagrain Iberica. Variety Helen B was obtained by Advanta; authorized the 11/08/2005; now commercialized by Limagrain Iberica. The insert integrated into the maize genome contains: Cauliflower Mosaic Virus 35S promoter + maize HSP70 intron (partial) + synthetic sequence coding for the Bacillus thuringensis CRYIA(b) insecticidal protein (truncated).

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Differences in the transcriptomes of organically and conventionally grown potatoes.

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Project description:The use of profiling techniques such as transcriptomics, proteomics, and metabolomics has been proposed to improve the detection of side effects of plant breeding processes. This paper describes the construction of a food safety-oriented potato cDNA microarray (FSPM). Microarray analysis was performed on a well-defined set of tuber samples of two different potato varieties, grown under different, well-recorded environmental conditions. Data were analyzed to assess the potential of transcriptomics to detect differences in gene expression due to genetic differences or environmental conditions. The most pronounced differences were found between the varieties Sante and Lady Balfour, whereas differences due to growth conditions were less significant. Transcriptomics results were confirmed by quantitative PCR. Furthermore, the bandwidth of natural variation of gene expression was explored to facilitate biological and/or toxicological evaluation in future assessments. Keywords: experiment with factorial design factorial design; 2 potato cultivars (Sante, Lady Balfour); 2 fertilizers (dairy manure compost, chicken manure pellets); 3 plant protection treatments (copper oxychloride, comcat, water), 3 biological replicates, 48 samples

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Maize grown under normal field conditions vs under 60% shade or under 2X density

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Field test for diagnostic P deficiency genes

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Expression data from Zea mays cultivars Tietar and DKC 6575

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