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Transcription profiling of Zea mays leaves from variety MON810 (Helen Bt) grown in agricultural fields at 2 levels of nitrogen fertilization

ABSTRACT: Comparison of the transcriptome profiles of a widely commercialized MON810 maize variety (HelenBt) at two levels of nitrogen fertilization. Variety Helen Bt; obtained by Advanta; authorized the 11/08/2005; now commercialized by Limagrain Iberica. Conventional nitrogen fertilization compared to not nitrogen fertilization during the season.

ORGANISM(S): Zea mays

SUBMITTER: Maria Pla

PROVIDER: E-MEXP-2368 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcription profiling of Zea mays leaves from variety Helen grown in agricultural fields at 2 levels of nitrogen fertilization

Project description:Comparison of the transcriptome profiles of a widely commercialized maize variety (Helen) at two levels of nitrogen fertilization. Conventional nitrogen fertilization compared to not nitrogen fertilization during the season.

2010-09-21 | E-MEXP-2367 | biostudies-arrayexpress

Transcription profiling of Zea mays leaves from MON810 (Helen Bt) and its near-isogenic couterpart (Helen) grown in agricultural fields

Project description:Comparison of the transcriptome profiles of a widely commercialized maize MON810 variety and its non-GM near-isogenic counterpart grown in agricultural fields. The Helen variety was commercialized by Limagrain Iberica. Variety Helen B was obtained by Advanta; authorized the 11/08/2005; now commercialized by Limagrain Iberica. The insert integrated into the maize genome contains: Cauliflower Mosaic Virus 35S promoter + maize HSP70 intron (partial) + synthetic sequence coding for the Bacillus thuringensis CRYIA(b) insecticidal protein (truncated).

2010-09-21 | E-MEXP-2366 | biostudies-arrayexpress

Transcription profiling of Zea mays leafs of MON810 (Helen Bt) and near-isogenic commercial varieties (Helen) gorwn in low nitrogen conditions

Project description:Comparison of the transcriptome profiles of a widely commercialized maize MON810 variety and its non-GM near-isogenic counterpart subjected to low N fertilization farming practices

2010-09-21 | E-MEXP-2364 | biostudies-arrayexpress

Differences in the transcriptomes of organically and conventionally grown potatoes.

2011-12-31 | E-MTAB-605 | biostudies-arrayexpress

Differences in the transcriptomes of organically and conventionally grown potatoes.

Project description:Microarray analysis was performed on a well-defined set of potato tuber samples grown under different, well-recorded environmental conditions. The data were analysed to assess the potential of transcriptomics to detect differences in gene expression as a result of these environmental conditions. Differences were found for both factors, both in PCA and in ANOVA analysis. Factorial design; 1 potato cultivar (Sante); 2 fertilizers (organic, conventional); 2 crop protection treatments (organic, conventional), 4 biological replicates, 16 samples. Raw data files: columns 1 - 11 is Cy3, 12 - 21 is Cy5

2012-01-31 | E-GEOD-27662 | biostudies-arrayexpress

Transcription profiling of developing field grown barley grain in response different nitrogen fertilizer regime

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2008-11-19 | E-MEXP-1013 | biostudies-arrayexpress

Transcriptome analysis of potato tubers: Effects of different agricultural practices

Project description:The use of profiling techniques such as transcriptomics, proteomics, and metabolomics has been proposed to improve the detection of side effects of plant breeding processes. This paper describes the construction of a food safety-oriented potato cDNA microarray (FSPM). Microarray analysis was performed on a well-defined set of tuber samples of two different potato varieties, grown under different, well-recorded environmental conditions. Data were analyzed to assess the potential of transcriptomics to detect differences in gene expression due to genetic differences or environmental conditions. The most pronounced differences were found between the varieties Sante and Lady Balfour, whereas differences due to growth conditions were less significant. Transcriptomics results were confirmed by quantitative PCR. Furthermore, the bandwidth of natural variation of gene expression was explored to facilitate biological and/or toxicological evaluation in future assessments. Keywords: experiment with factorial design factorial design; 2 potato cultivars (Sante, Lady Balfour); 2 fertilizers (dairy manure compost, chicken manure pellets); 3 plant protection treatments (copper oxychloride, comcat, water), 3 biological replicates, 48 samples

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Maize grown under normal field conditions vs under 60% shade or under 2X density

Project description:These experiments were to investigate changes in gene expression associated with maize competition for light when grown at double normal population density or under 60% shaded conditions as opposed to when maize is grown under normal field conditions. Three biological replicates (collected from separate field plots) comprised of pooled samples of 4 plants from each treatment were hybridized in a rolling circle dye swap hybridization screen.

2008-12-04 | E-GEOD-13768 | biostudies-arrayexpress

Field test for diagnostic P deficiency genes

Project description:Field test for diagnostic P deficiency genes

2012-04-12 | E-MTAB-634 | biostudies-arrayexpress

Expression data from Zea mays cultivars Tietar and DKC 6575

Project description:Maize transgenic event MON810, grown and commercialised worldwide, is the only cultivated GM event in EU. Maize MON810, variety DKC6575, and the corresponding near-isogenic Tietar were studied in different growing conditions, to assess their behaviour in response to drought. Profiling gene expression in water deficit regimes and in generalised water stress showed an up-regulation of different stress- responsive genes. A greater number of differentially expressed genes was observed in Tietar rather than in DKC6575, with genes belonging to transcription factor families and genes encoding HSPs, LEAs and detoxification enzymes. Since these genes have been from literature, indicated as typical of stress responses, their activation in Tietar rather than in DKC6575 may be reminiscent of a more efficient water stress response. DKC6575 was also analysed for the expression of the transgene CryIAb (encoding for the delta-endotoxin insecticidal protein) in water limiting conditions. In all the experiments the CryIAb transcript was not influenced by water stress, but expressed at a constant level. This suggests that though a different pattern of sensitivity to stress, the transgenic variety maintains the same expression level for the transgene. The Maize Oligonucleotide microarray was used to measure differences in gene expression levels under drought stress in DKC6575 and in the near-isogenic variety Tietar growing in the field. The gene expression profile of each variety was evaluated at T1 and at T2 for samples fully irrigated and at T2 for samples with irrigation deficit.

2015-02-23 | E-GEOD-59533 | biostudies-arrayexpress

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