Transcription profiling of E. coli NMB0419 mutants to investigate the role of this tetratricopeptide-repeats-encoding gene in bacterial adherence to human epithelial cells

ABSTRACT: The gene NMB0419 of Neisseria meningitidis strain MC58 encodes a putative tetratricopeptide repeats (TPR) containing protein, which was implicated in respiratory epithelial invasion. We have accordingly sought a role for NMB0419 in meningococcal adherence and invasion of human epithelial cells using Escherichia coli surrogates. In trans expression of NMB0419 promoted adherence of the E. coli strain to human epithelial cells, which showed a unique aggregative adherence pattern that was observed for pathogenic E. coli. This adherence was totally abolished by addition of D-mannose, suggesting that formation of type 1 pili on the surface of E. coli strain was solely responsible for the adherence and was facilitated by the expression of NMB0419. This was further supported by rigid pilus structu

ORGANISM(S): Escherichia coli

SUBMITTER: Ming-Shi Li 

PROVIDER: E-MEXP-2485 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress