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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of traps and vegetative hyphae of the nematophagous fungus Monacrosporium haptotylum

ABSTRACT: A microarray analysis was conducted to investigate transcriptional differences between traps and vegetative hyphae of the nematophagous fungus Monacrosporium haptotylum. Our goal was to identify genes that show differential regulation in the two different cell types; 1) traps (knobs) and 2) vegetative hyphae (mycelium) grown in the same medium. The entire design involved nine slides; four of these were hybridisations of knobs versus mycelium and five were mycelium against mycelium, 10 labelled extracts, and 3 biological replicates. The microarray experiments were designed as two-sample comparisons (i.e. knobs versus mycelium or mycelium versus mycelium) using three independent biological replicates, which also included technical and dye-swapped control hybridisations.

ORGANISM(S): Monacrosporium haptotylum

SUBMITTER: Csaba Fekete

PROVIDER: E-MEXP-250 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Comparison of gene expression in trap cells and vegetative hyphae of the nematophagous fungus Monacrosporium haptotylum.

Ahrén Dag D Tholander Margareta M Fekete Csaba C Rajashekar Balaji B Friman Eva E Johansson Tomas T Tunlid Anders A

Microbiology (Reading, England) 20050301 Pt 3

Nematode-trapping fungi enter the parasitic stage by developing specific morphological structures called traps. The global patterns of gene expression in traps and mycelium of the fungus Monacrosporium haptotylum were compared. The trap of this fungus is a unicellular spherical structure called the knob, which develops on the apex of a hyphal branch. RNA was isolated from knobs and mycelium and hybridized to a cDNA array containing probes of 2822 EST clones of M. haptotylum. Despite the fact tha ...[more]

PMID: 15758225

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