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Dataset Information

Comparative genomic hybridization of rat SJL sub-strains of an experimental autoimmune encephalomyelitis (EAE) rodent model of Multiple Sclerosis (MS)

ABSTRACT: Copy number variation in two SJL sub-strains of an experimental autoimmune encephalomyelitis (EAE) rodent model of Multiple Sclerosis (MS)

ORGANISM(S): Mus musculus

SUBMITTER: Lucy Osborne

PROVIDER: E-MEXP-2534 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Substrain differences reveal novel disease-modifying gene candidates that alter the clinical course of a rodent model of multiple sclerosis.

deLuca Leslie E Summers LE Pikor Natalia B NB O'Leary Jennifer J Galicia-Rosas Georgina G Ward Lesley A LA Defreitas Dustin D Finlay Trisha M TM Ousman Shalina S SS Osborne Lucy R LR Gommerman Jennifer L JL

Journal of immunology (Baltimore, Md. : 1950) 20100219 6

Experimental autoimmune encephalomyelitis (EAE) is a rodent model of multiple sclerosis that is executed in animals by immunization with myelin Ag in adjuvant. The SJL/J autoimmune-prone strain of mouse has been used to model relapsing-remitting multiple sclerosis. However, significant variations in peak scores, timing of onset, and incidence are observed among laboratories, with the postacute (relapse) phase of the disease exhibiting significant inconsistency. We characterized two substrains of ...[more]

PMID: 20173032

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Project description:Background Primitive brain tumors are the first cause of cancer-related death in children. Tumor cells with stem-like properties (TSCs), thought to account for tumorigenesis and therapeutic resistance, have been isolated from high-grade gliomas in adults. Whether TSCs are a common component of pediatric brain tumors and are of clinical relevance remains to be determined. Methodology/Principal findings Tumor cells with self-renewal properties were isolated with cell biology techniques from a majority of 55 pediatric brain tumors samples, regardless of their histopathologies and grades of malignancy (57% of embryonal tumors, 57% of low-grade gliomas and neuro-glial tumors, 70% of ependymomas, 91% of high-grade gliomas). The vast majority (10/12) of high-grade glioma-derived oncospheres sustained long-term self-renewal numbers akin to neural stem cells (>7 self-renewals), whereas cells with limited renewing abilities akin to neural progenitors dominated in all other tumor types. Regardless of tumor entities, the young age group was associated with self-renewal properties akin to neural stem cells (P=0.05, chi-square test). TSCs shared a complex molecular profile combining embryonic stem cell markers with elements controlling neural stem cell properties and epithelio-mesenchymal transitions. They were radio- and chemoresistant and formed aggressive tumors after intracerebral grafting. Survival analysis of the cohort showed an association between isolation of TSCs with long-term self-renewal abilities and a patients higher mortality rate (P = 0.022, log-rank test). Patients bearing cells with limited self-renewal properties constituted an intermediate group of survival but which did not reach statistical significance. Conclusions/Significance In brain tumors affecting adult patients, TSC have been isolated only from high-grade gliomas. In contrast, our data show that tumor cells with stem cell-like or progenitor-like properties can be isolated from a wide range of histological sub-types and grades of pediatric brain tumors. They suggest that cellular mechanisms fueling tumor development differ between adult and pediatric brain tumors.

Dataset Information

Comparative genomic hybridization of rat SJL sub-strains of an experimental autoimmune encephalomyelitis (EAE) rodent model of Multiple Sclerosis (MS)

Publications

Substrain differences reveal novel disease-modifying gene candidates that alter the clinical course of a rodent model of multiple sclerosis.

Similar Datasets

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