Transcription profiling of Arabidopsis seedlings grown under light or dark conditions in a time series
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ABSTRACT: Arabidopsis seedling were grown 3 days in complete darkness then put into normal light regime for 24 h Seedling were harvesrted at 15 min, 1, 2, 4, 8, 24h
Project description:The aim of this work was to analyse the expression of the Buchnera genes within different embryonic compartments (small, medium and large) as compared to the maternal bacteriocytes in the aphid Acyrtosiphon pisum.
Project description:Effects of the compound Pyrrolidine dithiocarbamate (PDTC) on the gene expression of Saccharomyces cerevisiae after incubation times of 1, 3 and 5 hours with 75uM PDTC.
Project description:Hybridisation of reference strains to the VirEp Staphylococcus aureus microarray, and characterisation of different S. aureus isolates from different locations and associated with different diseases.
Project description:Comparison between the multi-drug resistance Salmonella enteric serotype Newport strains from the US and the pan-susceptible strains from the UK
Project description:In order to determine genes that are differentially expressed during angiogenesis, Human Umbilical Vein Endothelial Cells (HUVEC) were cultured as 3D cultures undergoing tubulogenesis in a 3D fibrin matrix, or cultured as monolayers on top of a 3D fibrin matrix. RNA was then collected, reverse transcribed to cDNA and hybridized to glass slide oligo arrays containing 19k human genes. Differentially expressed genes in HUVECs undergoing tubulogenesis were then determined by comparing 2D to 3D culture samples.
Project description:C. albicans (strain CAI4-CIp10) was grown according to CRISP SOP. An overnight culture was started from a single colony in YPD-Tris,(100mM Tris.HCl) pH 7.4 and incubated overnight at 30C in shaking incubator. The next day, 500 ul culture was inoculated into 50 ml YPD-Tris, pH 7.4. The following day a fresh culture was inoculated in YPD-Tris to an OD600 of 0.2 and grown to OD600 of 0.8 at 30C in a shaking incubator. At this point the culture was split, diluted back to OD600 of 0.2 in fresh medium and stress agents added at time =0 (XS = 5mM H2O2, OS = 1M NaCl or a combination, OSXS). After 10 min the cultures were harvested by centrifugation and cell pellets flash frozen in liquid nitrogen. Three independent cultures were grown for RNA extraction for each isolate at each condition.<br><br>The control sample was obtained from cells with no stress agents added, harvested at time=0. RNA was extracted and transcript profiling carried out by microarray analysis using custom microarrays (Eurogentec).
Project description:Chlorophyll was removed from the veins of the C3 plant Arabidopsis by combining enhancer trap and RNAi approaches. Three independent transgenic lines were created. These were each compared to the parent line, J1511, in a microarray experiment.