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Transcription profiling of Arabidopsis plants with the chlorophyll removed by combining enhancer trap and RNAi approaches


ABSTRACT: Chlorophyll was removed from the veins of the C3 plant Arabidopsis by combining enhancer trap and RNAi approaches. Three independent transgenic lines were created. These were each compared to the parent line, J1511, in a microarray experiment.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Sophie Janacek 

PROVIDER: E-MEXP-1988 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Photosynthesis in cells around veins of the C(3) plant Arabidopsis thaliana is important for both the shikimate pathway and leaf senescence as well as contributing to plant fitness.

Janacek Sophie H SH   Trenkamp Sandra S   Palmer Ben B   Brown Naomi J NJ   Parsley Kate K   Stanley Susan S   Astley Holly M HM   Rolfe Stephen A SA   Paul Quick W W   Fernie Alisdair R AR   Hibberd Julian M JM  

The Plant journal : for cell and molecular biology 20090319 2


Cells associated with veins of C(3) species often contain significant amounts of chlorophyll, and radiotracer analysis shows that carbon present in the transpiration stream may be used for photosynthesis in these cells. It is not clear whether CO2 is also supplied to these cells close to veins via stomata, nor whether this veinal photosynthesis supplies carbon skeletons to particular metabolic pathways. In addition, it has not been possible to determine whether photosynthesis in cells close to v  ...[more]

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