Project description:Medicago wildtype and MtRSD mutant were subjected to nodulation and sample were harvested from susceptible zones of inoculated roots 0-8 days post innoculation
Project description:S. meliloti 1021FDC5 was grown at 30ºC in 20 ml of TY broth with shaking to late logarithmic phase (optical density at 600 nm = 1-1.2). After incubation, cells were pelleted, washed twice in MM and resuspended in 2 ml of the latter medium. For time course experiments in liquid, 0.5 ml of the inoculation culture was added to 50 ml of fresh MM. At various times, samples were removed for determining viable cells counts as well as for RNA isolation/preparation (7 and 14 hours). For experiments on plates, 20 ml of MM containing 0.7% (Semisolid) or 1.3% (Solid) agar was dispensed onto each Petri dish and allowed to gel. The plates were air dried at room temperature for 15 min. 0.1 ml of the inoculation culture was plated onto the surface of the plates and allowed to dry for 10 min. The plates were then inverted and incubated at 30ºC.
Project description:Transcriptome analysis in nodules of Medicago truncatula with customn 2.3K cDNA arrays.<br><br>Study of different nodule developmental stages in wild type.<br><br>Study of various mutants forming non-functional nodules.
Project description:Wild-type (Col-0) and mutant plants (adg1-1, tpt-2, adg1-1/tpt-2) were grown for 28 days under low light conditions and were then transferred to HL. Leaf samples for transcriptional profiling were taken at time 0 (before transfer to high light) and 4 hours and 48 hours (2 days) after transfer to high light.
Project description:Here we compare transcriptomic data generated using Affymetrix one-cycle (standard labelling protocol), two-cycle (small-sample protocol) and IVT-Express protocols with the Affymetrix ATH1 array using Arabidopsis root samples. Results obtained with each protocol are broadly similar. However, we show that there are 35 probe sets (of a total of 22810) that are misrepresented in the two-cycle data sets. Of these, 33 probe sets were classed as mis-amplified when comparisons of two independent publicly available data sets were undertaken.
Project description:Expression profile of meiosis in strain with Ndt80 inducible by addition of Estradiol (as in Carlile and Amon 2008), in three time points from addition of Estradiol.