Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Translation profiling of Methanococcoides burtonii to identify transcripts that interact with a recombinant archaeal MbRpoE/F heterodimer


ABSTRACT: The molecular machine that synthesizes RNA in Eucarya and Archaea, RNA polymerase, is composed of 11 or 12 subunits M-^V 9 or 10 that form the core holoenzyme, and a heterodimer formed from subunits E and F that associates with the core.

In this study we used a recombinant archaeal MbRpoE/F heterodimer to capture cellular mRNA and a custom Agilent microarray to determine which mRNA it binds. Transcripts bound by the heterodimer were identified through competitive hybridization of the total RNA obtained from Methanococcoides burtonii and the RNA obtained through the selection of the transcripts that interact with the MbRpoE/F heterodimer bound to the column.

ORGANISM(S): Methanococcoides burtonii

SUBMITTER: Stefano Campanaro 

PROVIDER: E-MEXP-2625 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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