Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling by array of Arabidopsis seedlings response to oscillations and prolonged increases in cytosolic Ca2+

ABSTRACT: Comparison of two types of calcium signature produced by application of voltage in Arabidopsis thaliana seedlings; namely oscillations or prolonged increase in cytosolic Ca2+ , each treatment is a biological dyeswap compared to untreated control

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Nigel Saunders

PROVIDER: E-MEXP-2659 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Transcription profiling by array of Arabidopsis seedlings response to transient and prolonged increases in cytosolic Ca2+

Project description:Comparison of the effect of different types of cytosolic Ca2+ elevations (measured by aequorin luminescence) in Arabidopsis seedlings. Calcium elevations are produced by electrical stimulation; namely transient (short, high amplitude) elevation and prolonged (lower amplitude, longer duration)- both were both biologically replicated. Biological replicates were processed as dye-swapped hybridisations.

2010-07-01 | E-MEXP-2661 | biostudies-arrayexpress

Transcription profiling by array of Arabidopsis seedlings response to three oscillating levels in cytosolic Ca2+

Project description:Comparison of 3 oscillating elevations in cytosolic Ca2+ (created using electrical stimulation and measured using aequorin luminescence) in Arabidopsis seedlings. Treatment 1; high frequency high amplitude osc., Treatment 2; high frequency low amplitude osc., Treatment 3; low frequency low amplitude osc. One biological sample per experiment processed as technical dye swaps against intreated control.

2010-08-01 | E-MEXP-2663 | biostudies-arrayexpress

Transcription profiling of Arabidopsis wild type and vin3.4 mutant seedlings exposed to low oxygen conditions

Project description:The effect of a 24 hour hypoxia (0.1% O2) treatment on wild-type (Col:FRI) seedlings and vin3.4 mutant seedlings, compared to control conditions. Compare wild-type control to vin3.4 control Compare wild-type control to wild-type 0.1% O2 Compare vin3.4 control to vin3.4 0.1% O2 Compare wild-type 0.1% O2 to vin3.4 0.1% O2

2009-06-01 | E-MEXP-2073 | biostudies-arrayexpress

transcriptomic analysis of phosphatin effect-Phosphatin effect on Arabidopsis

Project description:cea11-02_phosphatin - transcriptomic analysis of phosphatin effect - Identification of transcriptomic modifications promoted by phosphatin (AC6) (a molecule mimicking Pi addition effects on Pi starved plants). - 40 ÂµM Phosphatin was added to MS (diluted 10 times) containing 20ÂµM phosphate medium and controls were grown on MS (diluted 10 times) containing 20ÂµM phosphate or supplemented with 500 ÂµM of Pi. For each ARN extraction their was 3 plates containing each 10 seedlings grown 13 days in vertical petri dishes. 6 dye-swap - treated vs untreated comparison

2013-04-11 | E-GEOD-45937 | biostudies-arrayexpress

Transcription profiling of Arabidopsis thaliana seedlings using different Affymetrix RNA labelling protocols

Project description:Here we compare transcriptomic data generated using Affymetrix one-cycle (standard labelling protocol), two-cycle (small-sample protocol) and IVT-Express protocols with the Affymetrix ATH1 array using Arabidopsis root samples. Results obtained with each protocol are broadly similar. However, we show that there are 35 probe sets (of a total of 22810) that are misrepresented in the two-cycle data sets. Of these, 33 probe sets were classed as mis-amplified when comparisons of two independent publicly available data sets were undertaken.

2010-03-13 | E-MEXP-2608 | biostudies-arrayexpress

Transcription profiling of Arabidopsis seedlings overexpressing ERF6, a transcription factor of the ethylene response factor family

Project description:Microarray analyses were performed on Arabidopsis thaliana plants overexpressing ERF6. In this way it was hoped that any changes in the Arabidopsis transcriptome caused by overexpression would provide clues as to the role this gene plays in vivo. Arabidopsis thaliana plants were transformed with 35S::ERF6 or the empty vector pK2GW7 (used as a control). Total RNA was extracted from untreated 10-day old seedlings. Three separate arrays were performed (the RNA for each slide having been extracted from an independent biological replicate).

2010-12-31 | E-MTAB-435 | biostudies-arrayexpress

Transcription profiling of Arabidopsis seedlings over-expressing ERF5 or ERF6, transcription factors of the ethylene response factor family

Project description:Microarray analyses were performed on Arabidopsis thaliana plants over-expressing either AtERF5 or AtERF6, which are both transcription factors of the ethylene response factor (ERF) family. In this way it was hoped that any changes in the Arabidopsis transcriptome caused by their over-expression would provide clues as to the role(s) these genes play in vivo. Arabidopsis thaliana plants were transformed with either 35S::AtERF5, 35S::AtERF6 or the empty vector pK2GW7 (used as a control). Total RNA was extracted from untreated 10-day old seedlings. Three separate arrays were performed for each of the two over-expressors, (the RNA for each slide having been extracted from an independent biological replicate).

2010-12-31 | E-MEXP-2092 | biostudies-arrayexpress

Transcription profiling by array of Arabidopsis wild type and an ARF7/ARF19 double knockout mutant roots dissected into 5 developmental zones

Project description:Arabidopsis seedlings, of both wild-type and an ARF7/ARF19 double knockout mutant, were grown to 7 days post-germination. The roots were then dissected into 5 developmental zones, the meristem, early elongation zone, late elongation zone, mature root and lateral root zone. The sections then underwent transcriptional profiling to identify processes and regulatory events specific and in common to the zones.

2011-04-13 | E-MEXP-2912 | biostudies-arrayexpress

Transcription profiling of Arabidopsis roots after treatment with rhizotoxic ions Al, Cu, Cd and NaCl

Project description:Comparison of gene expression responses to rhizotoxic ions (Al, Cu, Cd and NaCl) in Arabidopsis roots.

2009-05-04 | E-MEXP-1907 | biostudies-arrayexpress

Transcription profiling of Arabidopsis seedlings overexpressing ERF5, a transcription factor of the ethylene response factor family

Project description:Microarray analyses were performed on Arabidopsis thaliana plants overexpressing ERF5. In this way it was hoped that any changes in the Arabidopsis transcriptome caused by overexpression would provide clues as to the role this gene plays in vivo. Arabidopsis thaliana plants were transformed with 35S::ERF5 or the empty vector pK2GW7 (used as a control). Total RNA was extracted from untreated 10-day old seedlings. Three separate arrays were performed (the RNA for each slide having been extracted from an independent biological replicate).

2010-12-31 | E-MTAB-436 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data