BIGenomics_hpv_dilution_series
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ABSTRACT: A dilution series experiment to determine the sensitivity of a microarray method for human papillomavirus genotyping. HPV genotyping is based on multiplex PCR (PGMY-t primers) followed by ligation step where two probes are ligated together if a matching template is present in the mixture. The ligated probes are then detected on microarray. Plasmids containing the full genome of HPV 16 or 18 were used in the experiment. The concentrations were 1000, 100, 10 ,1, 0.1 and 0 femtograms of plasmid as a template in HPV PCR reaction.
ORGANISM(S): Human papillomavirus
SUBMITTER: Jarmo Ritari
PROVIDER: E-MEXP-3477 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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