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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling by array of mouse CD4+ GFP+ (tregs) and CD4+ GFP- (Teffs) cells treated with IL-2

ABSTRACT: To obtain the molecular signature of the effect that the IL-2 therapeutic dose induces on T cells in vivo, we performed gene array analysis. Since it was critical to perform this analysis on highly purified Tregs and Teffs, and also because activated Teffs can acquire CD25 expression, we used GFP-Foxp3 knock-in mice which allow for the purification of Tregs and Teffs based on the GFP expression. Illumina gene array was carried out on FACS sorted CD4+ GFP+ (Tregs) and CD4+ GFP- (Teffs) of mice that received either PBS or the curative schedule of IL-2, 2 hours after the last injection.

ORGANISM(S): Mus musculus

SUBMITTER: Nicolas Cagnard

PROVIDER: E-MEXP-2689 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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