Transcription profiling by array of rice plants following dehydration treatment
Ontology highlight
ABSTRACT: Wild-type rice plants (O. sativa L. cv. Nipponbare) were grown in plastic pots filled with nutrient soil for 2 weeks under flooded lowland conditions and a 12 h/12 h light/dark cycle (50 ± 10 ?mol photons/m2/s) at 28°C (day) and 25°C (night). For dehydration treatment, two-week-old plants were incubated for 3 days without watering. The soil moisture content was 15.6% on day 3 of dehydration.
Project description:Effect of single or double knock-out mutation of subclass III SnRK2 protein kinases in Arabidopsis after 6 h of treatment with dehydration stress.
Project description:Total RNA was isolated with RNAiso regent (Takara) from 12-day-old seedlings of the areb1 areb2 abf3 triple mutant and WT plants grown on GM agar plates with stress treatment of dehydration for 6 h.
Project description:Expression profiles of the wild-type Arabidopsis (Col) and srk2dei triple mutant plants in response to ABA, NaCl, and dehydration treatments.<br><br>
Project description:Gene expression profiles in transgenic rice plants that overexpressed OsNAC5, driven by the maize ubiquitin promoter (UBIpro::OsNAC5).
Project description:Total RNA was isolated with RNAiso regent (Takara) from 12-day-old seedlings of the areb1 areb2 abf3 triple mutant and WT plants grown on GM agar plates.
Project description:Wild-type rice plants (O. sativa L. cv. Nipponbare) were grown in plastic pots filled with nutrient soil for 2 weeks under flooded lowland conditions and a 12 h/12 h light/dark cycle (50 M-1 10 ?mol photons/m2/s) at 28M-0C (day) and 25M-0C (night). For cold treatment, two-week-old plants were transferred from 28M-0C to 10M-0C and incubated for 1 day.
Project description:Total RNA was isolated with RNAiso regent (Takara) from 12-day-old seedlings of the areb1 areb2 abf3 triple mutant and WT plants grown on GM agar plates with or without 50 µM ABA for 6 h.
Project description:Total RNA was isolated with RNAiso regent (Takara) from 12-day-old seedlings of the areb1 areb2 abf3 triple mutant and WT plants grown on GM agar plates with stress treatment of 250 mM NaCl for 6 h.
Project description:Transcriptional profiling of roots and hypocotyls of soybean comparing control untreated 2-d-old seedlings with flooding treated 2-d-old seedlings.
Project description:Microarray experiments of the abi5 seeds were performed by analyzing the samples obtained from two different alleles (both for wild-type and mutant). For the analysis of gene expression profiles in abi5-1 seeds, RNA from Ws seeds was used. For the analysis of gene expression profiles in abi5-7 seeds, RNA from Col seeds was used.