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Transcription profiling by array of mouse wild type and E-cadherin knockout embryonic stem cells grown with or without cytokine leukemia inhibitory factor

ABSTRACT: We previously observed that E-cadherin knockout embryonic stem cells do not differentiate in the absence of the cytokine leukemia inhibitory factor (LIF) (Soncin et al., 2009, Stem Cells 27 p2069-2080). This experiment was designed to compare the transcription profile of E-cadherin KO ES cells cultured in the presence and absence of LIF. We also compared E-cadherin KO ES cells with wild type D3 ES cells grown in the presence of LIF.

ORGANISM(S): Mus musculus

SUBMITTER: Leo Zeef

PROVIDER: E-MEXP-2836 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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E-cadherin acts as a regulator of transcripts associated with a wide range of cellular processes in mouse embryonic stem cells.

Soncin Francesca F Mohamet Lisa L Ritson Sarah S Hawkins Kate K Bobola Nicoletta N Zeef Leo L Merry Catherine L R CL Ward Christopher M CM

PloS one 20110714 7

<h4>Background</h4>We have recently shown that expression of the cell adhesion molecule E-cadherin is required for LIF-dependent pluripotency of mouse embryonic stem (ES) cells.<h4>Methodology</h4>In this study, we have assessed global transcript expression in E-cadherin null (Ecad-/-) ES cells cultured in either the presence or absence of LIF and compared these to the parental cell line wtD3.<h4>Results</h4>We show that LIF has little effect on the transcript profile of Ecad-/- ES cells, with s ...[more]

PMID: 21779327

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