Project description:Analysis of genes enriched in MIXL1+ sorted cells during primitive streak induction identified Apelin receptor (APLNR), a highly conserved member of the G-protein coupled receptor family. Depending upon the growth factor conditions used for differentiation, APLNR+ cells identified both posterior mesodermal and anterior mesendodermal components of the primitive streak. APLNR+ cells isolated from mesodermal differentiated cultures were enriched in hematopoietic blast colony forming cells (Bl-CFCs) and the addition of Apelin peptide enhanced the frequency and growth of both hematopoietic colonies and hESC-derived endothelial cells. These studies identified APLNR as a marker of primitive streak-like cells differentiated from hESCs and defined a novel role for Apelin as a regulator of early human hematopoiesis.
Project description:chromatin immunoprecipitations were performed in mouse haematopoietic and endothelial cell lines using an anti-H3 trimethyl lysine 4 antibody
Project description:Transcription profiling of human mesenchymal cells (MSCs) after ex vivo expansion under culture conditions supporting extensive cell proliferation. Highly efficient cell expansion within short time (~40 days) and comparison of gene expression profiles from MSCs after primary seeding, defined as early passage versus MSCs after a minimum of 17 (max 34) population doublings, defined as late passage. Combined data of passage 1 and 2 was compared to passage 0 (zero) as reference
Project description:Lotus japonicus is a perennial legume with a small diploid genome that has been adopted as a model species for legume genetics and genomics. With the genome sequence as a backdrop (Sato et al. 2008), we have generated a gene expression atlas that provides a global view of gene expression in all major organ systems of this species, including nodule and seed development.