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Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling by array of human peripheral blood lymphocytes or haemopoietic stem cells at the time of transduction with a gammaretroviral vector

ABSTRACT: Expression profile comparison of peripheral blood lymphocytes (PBL) or haemopoietic stem cells (HSC) at the time of transduction with a gammaretroviral vector. The analysis is performed in the context of two different gene therapy clinical trials (Recchia et al, 2006; Aiuti et al, 2007) based on autologous infusion of human T cells or CD34+ haemopoietic progenitor cells gene-corrected with an moloney leukemia virus-derived vector (MLV-vector). T cells and CD34+ cells were isolated from patients and pre-stimulated in vitro with cytokines prior to transduction with the vector and re-infusion in patient. The expression profiles of T cells and CD34+ cells were analysed by microarray just after cytokine stimulation, providing information on the transcriptional activity of these two target cells at the time of transduction.

ORGANISM(S): Homo sapiens

SUBMITTER: Luca Biasco

PROVIDER: E-MEXP-2983 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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