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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Spheres culture from lung adenocarcinoma pleural effusions

ABSTRACT: Briefly, we analyzed gene expression profiles in adherent cultures compared to sphere cultures from malignant pleural effusions (MPEs). MPEs could represent an opportunity to culture a wide variety of cancer cells from different donors. In this study, we set up culture conditions for cancer cells deriving from MPEs of several patients affected by the most frequent form of lung cancer, namely the subset of non small cell lung cancers (NSCLC) classified as Lung Adenocarcinomas, to show that MPEs are an excellent source of tumor initiating cells for the study of lung adenocarcinoma.

ORGANISM(S): Homo sapiens

SUBMITTER: Pietro Zoppoli

PROVIDER: E-MEXP-3025 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Spheres derived from lung adenocarcinoma pleural effusions: molecular characterization and tumor engraftment.

Mancini Rita R Giarnieri Enrico E De Vitis Claudia C Malanga Donatella D Roscilli Giuseppe G Noto Alessia A Marra Emanuele E Laudanna Carmelo C Zoppoli Pietro P De Luca Pasquale P Affuso Andrea A Ruco Luigi L Di Napoli Arianna A Mesiti Giuseppe G Aurisicchio Luigi L Ricci Alberto A Mariotta Salvatore S Pisani Lara L Andreetti Claudio C Viglietto Giuseppe G Rendina Erino A EA Giovagnoli Maria Rosaria MR Ciliberto Gennaro G

PloS one 20110718 7

Malignant pleural effusions (MPEs) could represent an excellent source to culture a wide variety of cancer cells from different donors. In this study, we set up culture conditions for cancer cells deriving from MPEs of several patients affected by the most frequent form of lung cancer, namely the subset of non small cell lung cancers (NSCLC) classified as Lung Adenocarcinomas (AdenoCa) which account for approximately 40% of lung cancer cases. AdenoCa malignant pleural effusions gave rise to in v ...[more]

PMID: 21789168

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