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Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human embryonic stem (ES) cells. Undifferentiated cells of different passage numbers (p19 and p128) were vs cells differentiated in hanging drops for 5 days (d5 embryoid bodies) or expanded on gelatin coated dishes for a further 9 days (d14 embryoid bodies)

ABSTRACT: Undifferentiated cells of different passage numbers (p19 and p128) were compared to cells differentiated in hanging drops for 5 days (d5 embryoid bodies) or expanded on gelatin coated dishes for a further 9 days (d14 embryoid bodies).

ORGANISM(S): Homo sapiens

SUBMITTER: Elizabeth Ng

PROVIDER: E-MEXP-303 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptional profiling of mouse and human ES cells identifies SLAIN1, a novel stem cell gene.

Hirst Claire E CE Ng Elizabeth S ES Azzola Lisa L Voss Anne K AK Thomas Tim T Stanley Edouard G EG Elefanty Andrew G AG

Developmental biology 20060320 1

We analyzed the transcriptional profiles of differentiating mouse embryonic stem cells (mESCs) and show that embryoid bodies (EBs) sequentially expressed genes associated with the epiblast, primitive streak, mesoderm and endoderm of the developing embryo, validating ESCs as a model system for identifying cohorts of genes marking specific stages of embryogenesis. By comparing the transcriptional profiles of undifferentiated ESCs to those of their differentiated progeny, we identified 503 mESC and ...[more]

PMID: 16546155

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